In this lab, many materials were used including, EcoRl restriction enzyme, DNA from the crime scene and subjects 1 through 5, centrifuge, water bath, DNA loading dye, Electrophoresis chamber, TAE buffer, and a fast blast stain. The EcoRl restriction enzyme is the enzyme that was used in order to cut the DNA at specific sites into many separate fragments. A centrifuge is a machine that uses a rotating container inside it in order to force all of the liquids to the bottom of the test tubes within the container. A water bath is a heated bath of water which is usually set at a specific temperature that is used to heat up substances. The DNA loading dye was used in order to visually see the DNA against the clear buffer solution and the clear agarose
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This is known because when the DNA of all suspects and the crime scene are put through Agarose gel electrophoresis the DNA fragments can be measured. The original crime scene showed three DNA fragment bands for its results. The first band was 37 mm, which means that it 4,108 base pairs. Suspect three’s first band was 36 mm and had 5,000 base pairs. The crime scene’s second band was 40 mm and 3,102 base pairs. Suspect three’s second band was 39 mm and was 3,500 base pairs. The crime scene’s last and final third band was 44 mm and was 2,407 base pairs. Suspect three’s last and final third band was 42 mm and was 2,800 base pairs. Even though the two are not identical, they are close enough to be able to say that they came from the source. This person can now be convicted for the crime they have committed with the evidence provided …show more content…
PCR is polymerase chain reaction which is the process of rapid copying of genes of interest, it’s like photocopying DNA. It is done by heating the DNA in order to separate the strands aka denature the DNA. While the DNA is being denatured a heat resistant DNA polymerase from a thermophilic bacteria. This bacteria lives in the hot springs of Yellowstone National Park and is called Thermus aquaticus. After that is completed, primers are added to the strands in a process known as annealing. This cycle continues and can create billions of DNA copies in just over a few hours, which can turn a small sample of evidence that is too small to be used into a large enough sample that can be tested by everyone with plenty left over. Gel electrophoresis can also be used in other situations that are not crime scenes. It was used after the attack on the World Trade Center in 2001, to identify the incinerated victims in order to notify their families and loved ones. Gel electrophoresis is also used in kinship disputes like determining if he’s really the father or if that’s really the child. It can also be used in testing medications and vitamins to see if they really are what they say they
This is known because when the DNA of all suspects and the crime scene are put through Agarose gel electrophoresis the DNA fragments can be measured. The original crime scene showed three DNA fragment bands for its results. The first band was 37 mm, which means that it 4,108 base pairs. Suspect three’s first band was 36 mm and had 5,000 base pairs. The crime scene’s second band was 40 mm and 3,102 base pairs. Suspect three’s second band was 39 mm and was 3,500 base pairs. The crime scene’s last and final third band was 44 mm and was 2,407 base pairs. Suspect three’s last and final third band was 42 mm and was 2,800 base pairs. Even though the two are not identical, they are close enough to be able to say that they came from the source. This person can now be convicted for the crime they have committed with the evidence provided …show more content…
PCR is polymerase chain reaction which is the process of rapid copying of genes of interest, it’s like photocopying DNA. It is done by heating the DNA in order to separate the strands aka denature the DNA. While the DNA is being denatured a heat resistant DNA polymerase from a thermophilic bacteria. This bacteria lives in the hot springs of Yellowstone National Park and is called Thermus aquaticus. After that is completed, primers are added to the strands in a process known as annealing. This cycle continues and can create billions of DNA copies in just over a few hours, which can turn a small sample of evidence that is too small to be used into a large enough sample that can be tested by everyone with plenty left over. Gel electrophoresis can also be used in other situations that are not crime scenes. It was used after the attack on the World Trade Center in 2001, to identify the incinerated victims in order to notify their families and loved ones. Gel electrophoresis is also used in kinship disputes like determining if he’s really the father or if that’s really the child. It can also be used in testing medications and vitamins to see if they really are what they say they