Ebola is a virus that is very deadly. Four out of five strains can make people sick. While inside of the body it is possible to do the following things: kills cells, full bleeding internally, wrecks the immune system, and harms every organ in the body. It is very infrequent to catch Ebola. The only way that people can get infected with the disease is from direct contact with fluids from those who are already infested with Ebola. Ebola was first recognized in 1976. “The name came from a river in the Democratic Republic of the Congo (formerly Zaire) in Africa, where the virus was first recognized” says Arthur Schoenstadt, MD. There have been 18 definite outbreaks of the illness since 1976. When it was first recognized there have been outbreaks
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For the diagnosis, specimens for molecular findings should preferably be taken in for help when a patient shows symptoms that meet the case description of Ebola virus disease. If specimens are collected less than 3 days after onset of symptoms, more specimens will be needed if the test result on the first specimen is negative. The second specimen should be collected at no less than 48 hours after the first specimen. Laboratory biosafety recommendations are next of the diagnosis. Virus isolation should be done in an extreme containment BSL4 laboratory. Safeguard and secure handling and storage of the virus separates and other specimens from accidental or deliberate release. The inactivation of specimens, reliant on the detection protocol used, should be completed under BSL3 conditions. For non-inactivated samples, RT PCR and enzyme-linked immunosorbent assay testing can be performed at a BSL3 laboratory. If samples have been inactivated (i.e. cell lysis) RT PCR and ELISA testing can be performed at a BSL2 laboratory. Laboratory diagnosis: Laboratory results should …show more content…
Laboratory-established cases has to test positive for the presence of the Ebola virus, either by detection of virus RNA by RT-PCR, and/or by detection of Ebola antigen by a precise Antigen detection test, and/or by detection of Immunoglobulin M (IgM) antibodies directed against Ebola. Two negative RT PCR test results, at least 48 hours apart, are required for a clinically asymptomatic patient to be discharged from hospital. After that step is completed, you ship the specimen out to observe for EVD. For countries without the capacity to test clinical specimens from cases under investigation: Clinical specimens are to be shipped as Category A, using proper packaging, markings and documentation. Preceding discussion with the recipient laboratory is needed to arrange appropriate shipment and processing of the specimen. Specimen details have to be sent to the recipient laboratory. Patients with Ebola virus disease should receive care in selected treatment centers and by clinicians trained to care for ill patients. Treating patients with Ebola requires a multidisciplinary approach. All healthcare workers involved in the care of infected or potentially infected patients should use
For the diagnosis, specimens for molecular findings should preferably be taken in for help when a patient shows symptoms that meet the case description of Ebola virus disease. If specimens are collected less than 3 days after onset of symptoms, more specimens will be needed if the test result on the first specimen is negative. The second specimen should be collected at no less than 48 hours after the first specimen. Laboratory biosafety recommendations are next of the diagnosis. Virus isolation should be done in an extreme containment BSL4 laboratory. Safeguard and secure handling and storage of the virus separates and other specimens from accidental or deliberate release. The inactivation of specimens, reliant on the detection protocol used, should be completed under BSL3 conditions. For non-inactivated samples, RT PCR and enzyme-linked immunosorbent assay testing can be performed at a BSL3 laboratory. If samples have been inactivated (i.e. cell lysis) RT PCR and ELISA testing can be performed at a BSL2 laboratory. Laboratory diagnosis: Laboratory results should …show more content…
Laboratory-established cases has to test positive for the presence of the Ebola virus, either by detection of virus RNA by RT-PCR, and/or by detection of Ebola antigen by a precise Antigen detection test, and/or by detection of Immunoglobulin M (IgM) antibodies directed against Ebola. Two negative RT PCR test results, at least 48 hours apart, are required for a clinically asymptomatic patient to be discharged from hospital. After that step is completed, you ship the specimen out to observe for EVD. For countries without the capacity to test clinical specimens from cases under investigation: Clinical specimens are to be shipped as Category A, using proper packaging, markings and documentation. Preceding discussion with the recipient laboratory is needed to arrange appropriate shipment and processing of the specimen. Specimen details have to be sent to the recipient laboratory. Patients with Ebola virus disease should receive care in selected treatment centers and by clinicians trained to care for ill patients. Treating patients with Ebola requires a multidisciplinary approach. All healthcare workers involved in the care of infected or potentially infected patients should use