E. Colo Transformation Lab Report

Decent Essays
This lab was to understand the transformation of cells through the observation of the green fluorescent gene. E. coli cells were mixed with GFP plasmids, and then heat shocked so the plasmid would be able to infiltrate the E. coli cells and insert itself in the bacteria's own plasmid. Only half of the DNA was exposed to the pGFP, while the other half was used as a control. The control DNA was put onto two lysogeny broth agar plates, one plain, and one with ampicillin on it. The plain LB plate had substantial growth, since nothing was restricting the bacteria from growing. The plate with ampicillin, however had no growth since ampicillin kills bacteria. The DNA with the pGFP was also put on two LB agar plates one with ampicillin, and one with

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