After completing several thorough procedures to discover my unknown bacteria, bacteria #304, it was discovered to be Escherichia coli or for short E.coli. I can conclude that my answer is correct because several tests were conducted leading me to the specific bacteria. The first step taken to discover the unknown bacteria was a gram stain. A section of the unknown bacteria was taken and stained with crystal violet and safrinin so that the bacterium was visible under a microscope. The gram stain gave the opportunity to know whether the bacterium was gram negative or positive. This allowed me to narrow down my options knowing that my bacterium was gram negative, using the unknown identification key. After discovering whether my bacterium was gram
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A small amount of the bacteria was placed in to three diverse tubes, which had different carbohydrates. The bacteria produced acid and gas when placed in the tubes that had glucose, lactose and sucrose, however when placed in the tube of sucrose, acid was shown on the inside of the anaerobic part of the tube which caused no acid to be on the outside. The results given are typical for the E.coli carbohydrate fermentation. The last and final test that was done was a streak plate. After placing my bacteria was placed on an agar plate and endeavoring to isolate it, I was able to receive individual colonies and showed countable colony-forming units.My unknown bacteria’s, E.coli, morphology was that the bacteria were rod shaped. E.coli can be found plentifully in nature. E.coli can be found in the gut of endotherms, which are warm-blooded organisms. E.coli can also be found in the normal flora of the human stomach and allows numerous beneficial functions that include the production of vitamin (Nordquist 1). Most strains of E.coli are harmless, however the strain E.coli does have some pathogenic strains that cause
A small amount of the bacteria was placed in to three diverse tubes, which had different carbohydrates. The bacteria produced acid and gas when placed in the tubes that had glucose, lactose and sucrose, however when placed in the tube of sucrose, acid was shown on the inside of the anaerobic part of the tube which caused no acid to be on the outside. The results given are typical for the E.coli carbohydrate fermentation. The last and final test that was done was a streak plate. After placing my bacteria was placed on an agar plate and endeavoring to isolate it, I was able to receive individual colonies and showed countable colony-forming units.My unknown bacteria’s, E.coli, morphology was that the bacteria were rod shaped. E.coli can be found plentifully in nature. E.coli can be found in the gut of endotherms, which are warm-blooded organisms. E.coli can also be found in the normal flora of the human stomach and allows numerous beneficial functions that include the production of vitamin (Nordquist 1). Most strains of E.coli are harmless, however the strain E.coli does have some pathogenic strains that cause