The results show that the protein derived from the Drosophila cDNA sequence and the human protein share the same domain, also, there is a high degree of homology between the two proteins. Therefore, the Drosophila Melanogaster plasmid DNA sequence codes for a protein that has a human homologous involved in human physiology.
Researchers are still investigating the function of my protein in Drosophila Melanogaster. However, the function of the homologous protein in human is pretty clear. Indeed, the homologous protein is a mRNA-binding protein that takes part in translation elongation (3). It also plays an important role in the cell progression, mRNA decay and the maintenance of cell integrity. It regulates apoptosis, plays an important role …show more content…
Accordingly, Drosophila can be used a model organism to better understand diseases such as certain types of cancer and even infertility. That being said, in this kind of experiment where complicated procedures are involved, there is often room for errors. There were two main sources of errors in this experiment, the first one was the cDNA sequence that failed to yield significant results which lead to a bad chromatogram. The second source of error was seen in the gel electrophoresis as there were no DNA in the Plasmid B DNA lane. This happened because the PCR failed to amplify the cDNA sequence in the plasmid B DNA. In addition, the bad cDNA sequence might have been the result of cross contamination it could have occurred because our technique was not sterile. For, instance the pipettes were shared around the table, it was easy to measure the volume with a wrong pipette, or to pipette with a used pipette tip (we were required to use a fresh pipette tip for each dilution). Moreover, failure to set the right volume on the pipette counts as an error as well. We were required to wear gloves throughout the experiment, but at some point, some of us touched the pipettes, PCR tubes etc. with our bare hands. Thus, these mistakes might have affected our results. Therefore, it is imperative to conduct further researches in order to see whether or not we would have obtained the same results without these
Researchers are still investigating the function of my protein in Drosophila Melanogaster. However, the function of the homologous protein in human is pretty clear. Indeed, the homologous protein is a mRNA-binding protein that takes part in translation elongation (3). It also plays an important role in the cell progression, mRNA decay and the maintenance of cell integrity. It regulates apoptosis, plays an important role …show more content…
Accordingly, Drosophila can be used a model organism to better understand diseases such as certain types of cancer and even infertility. That being said, in this kind of experiment where complicated procedures are involved, there is often room for errors. There were two main sources of errors in this experiment, the first one was the cDNA sequence that failed to yield significant results which lead to a bad chromatogram. The second source of error was seen in the gel electrophoresis as there were no DNA in the Plasmid B DNA lane. This happened because the PCR failed to amplify the cDNA sequence in the plasmid B DNA. In addition, the bad cDNA sequence might have been the result of cross contamination it could have occurred because our technique was not sterile. For, instance the pipettes were shared around the table, it was easy to measure the volume with a wrong pipette, or to pipette with a used pipette tip (we were required to use a fresh pipette tip for each dilution). Moreover, failure to set the right volume on the pipette counts as an error as well. We were required to wear gloves throughout the experiment, but at some point, some of us touched the pipettes, PCR tubes etc. with our bare hands. Thus, these mistakes might have affected our results. Therefore, it is imperative to conduct further researches in order to see whether or not we would have obtained the same results without these