Animals and treatment
Male Wistar rats were purchased from National Institute of Nutrition, Hyderabad, India and the study was strictly done under the guidelines approved by the animal ethical Committee of CSIR-Institute of Genomics and Integrative Biology, Delhi, India. They were maintained under standard conditions (12/12 hour light/dark cycle at 25±5ºC). They were divided into five groups having six rats per cage and are allowed to acclimatization for 1 week.
Group 1(G1) - Healthy control (chow diet); Group 2 (G2) - HFD 150mg/kg body weight and the methanolic extract of Dracaena cinnabari resin 300mg/kg body weight; Group 3 (G3) - HFD 150mg/kg body weight and standard drug atorvastatin 30mg/kg body weight; Group 4 (G4) - HFD 150mg/kg body …show more content…
After that the treatment was followed by oral administration of methanolic extract of Dracaena cinnabari (300mg/kg/day); aqueous extract of pure compound curcumin (300 mg/kg/day) and standard drug atorvastatin (30mg/kg/day) daily for one month. After thirty days administration of the respective drug, the experimental and control rats were sacrificed and blood was collected into a vacutainer containing ethylene diamine tetraacetic acid (EDTA).
Sample preparation for two-dimensional gel electrophoresis
The blood samples were centrifuged at 1,500 rpm for 15 min at 4°C; plasma was isolated and stored at -80°C. Sample preparation was carried out by removing salt from the plasma by centrifugation and concentrated using 3 kDa amicon columns (Merck Millipore, USA) [16].
Two-dimensional gel …show more content…
Briefly, gels were placed in fixative (50% methanol and 12% acetic acid) for 1 h, soaked in 50% ethanol for 30 min followed by 30% ethanol for 30 min. The gels were then sensitized for 1 min in 2% solution of sodium sulfite (Na2SO3) followed by rinsing three times with Mili-Q for 20 sec and were stained for 20 min in staining solution [1M Silver nitrate (AgNO3) and formaldehyde (HCHO)]. After staining, gels were rinsed thrice for 20 sec in distilled water and developed for 5–10 min in a developer [sodium carbonate (Na2CO3) and formaldehyde (HCHO)] and observed for the appearance of intense bands for 1–2 min. The reaction was stopped with 6% acetic acid solution and scanned using chemiDocTM MP imaging system (Bio-Rad,
Male Wistar rats were purchased from National Institute of Nutrition, Hyderabad, India and the study was strictly done under the guidelines approved by the animal ethical Committee of CSIR-Institute of Genomics and Integrative Biology, Delhi, India. They were maintained under standard conditions (12/12 hour light/dark cycle at 25±5ºC). They were divided into five groups having six rats per cage and are allowed to acclimatization for 1 week.
Group 1(G1) - Healthy control (chow diet); Group 2 (G2) - HFD 150mg/kg body weight and the methanolic extract of Dracaena cinnabari resin 300mg/kg body weight; Group 3 (G3) - HFD 150mg/kg body weight and standard drug atorvastatin 30mg/kg body weight; Group 4 (G4) - HFD 150mg/kg body …show more content…
After that the treatment was followed by oral administration of methanolic extract of Dracaena cinnabari (300mg/kg/day); aqueous extract of pure compound curcumin (300 mg/kg/day) and standard drug atorvastatin (30mg/kg/day) daily for one month. After thirty days administration of the respective drug, the experimental and control rats were sacrificed and blood was collected into a vacutainer containing ethylene diamine tetraacetic acid (EDTA).
Sample preparation for two-dimensional gel electrophoresis
The blood samples were centrifuged at 1,500 rpm for 15 min at 4°C; plasma was isolated and stored at -80°C. Sample preparation was carried out by removing salt from the plasma by centrifugation and concentrated using 3 kDa amicon columns (Merck Millipore, USA) [16].
Two-dimensional gel …show more content…
Briefly, gels were placed in fixative (50% methanol and 12% acetic acid) for 1 h, soaked in 50% ethanol for 30 min followed by 30% ethanol for 30 min. The gels were then sensitized for 1 min in 2% solution of sodium sulfite (Na2SO3) followed by rinsing three times with Mili-Q for 20 sec and were stained for 20 min in staining solution [1M Silver nitrate (AgNO3) and formaldehyde (HCHO)]. After staining, gels were rinsed thrice for 20 sec in distilled water and developed for 5–10 min in a developer [sodium carbonate (Na2CO3) and formaldehyde (HCHO)] and observed for the appearance of intense bands for 1–2 min. The reaction was stopped with 6% acetic acid solution and scanned using chemiDocTM MP imaging system (Bio-Rad,