Dorsal Vs Western Blot

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Dorsal (dl) is a gene that codes for a protein which affects the development Drosophila cells by forming a gradient in the embryo. However, during development, the total amount of dl protein present in the each cell is about the same. What determines cell fate is the distribution of dl protein between the cell nucleus and cytoplasm. Researchers wanted to find out what would happen to the dl protein if they mutated the dorsal group genes, which they knew had an effect on the dorsoventral axis of the Drosophila embryo. They mutated one of the dorsal group genes from each embryo they tested and put extracts from each embryo on a Western blot. The Western blot showed the same bands for the mutants as for the wild-type embryo. This meant that

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