Dnase Assessment

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Professor, I found that in order to identify and distinguish any form of bacteria such as Serratia sp. and Enterobacter sp. a number of assessments should be conducted. Majority of bacteria’s has enzymes allowing it to break down nucleic acids and the bacteria and utilize the resulting nucleotides to produce their own nucleic acids. DNase is an enzyme that hydrolyzes DNA. The existence of DNase entails certain strains/species of bacteria and may be utilized for typing. A DNase assessment is determined by cultivation on an agar plate containing DNA. If the bacterium contains DNase and the bacterium grows overnight the DNA will have the ability to hydrolyze into the constituting nucleotide. HCI is diluted and poured into the plate. A streak appears

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