Explanation 1 (2 point)
Too little of a DNA sample was loaded into the agarose gel therefore the concentration of DNA was too low to allow amplification. To fix this increase your sample size of DNA. You may have also forgotten to load the dye into the sample, sample loading dye (which gives density to hold the DNA in the well) which is this case you made a mistake in loading the gel.
Explanation 2 (2 point)
Perhaps your primer did not anneal to the sequence because it is the wrong primer, or you have a contamination in your original sample. After you discover there is not a contamination and you have the proper primers, then you might want to optimize the annealing temperature for your primer. In addition, one could have forgotten to add the
Too little of a DNA sample was loaded into the agarose gel therefore the concentration of DNA was too low to allow amplification. To fix this increase your sample size of DNA. You may have also forgotten to load the dye into the sample, sample loading dye (which gives density to hold the DNA in the well) which is this case you made a mistake in loading the gel.
Explanation 2 (2 point)
Perhaps your primer did not anneal to the sequence because it is the wrong primer, or you have a contamination in your original sample. After you discover there is not a contamination and you have the proper primers, then you might want to optimize the annealing temperature for your primer. In addition, one could have forgotten to add the