Hypothesis: If I were to extract DNA without proper chemicals and equipment, the result DNA would become faulty or poor quality for further investigation
My hypothesis predicts that if we were to use improper or non-lab chemicals, the result DNA would become faulty and would result in an inadequate samples for further studies, it would seem that if we were to extract DNA without chemicals like lysis solution, concentrated salt solution, resuspenstion buffer, ethanol or isopropyl alcohol, it would result in poor DNA results.
Method one
Method one to create a perfect result and to extract the optimal DNA, I would believe that we would need centrifuge, sample tube, micropipettors, lysis solution, concentrated salt solution, resuspenstion buffer, …show more content…
We can get these cheek cells by just brushing the side of our mouth with a slab, but before we get the cells, we must rinse for 30 seconds to remove all the extra food or any other unwanted subjects. After we rinse then brushed the insides of the mouth to get the cheek cells, we can cut the end of the slab and put it into a sample tube. The first step into extracting the DNA is to mix the slab with some lysis solution, using the micropipettor we can add the desired amount of lysis solution, after adding the solution using …show more content…
Such as a saline solution (salted water), a clean glass, 1 tea spoon (5ml) of detergent, 3 tea spoon (15ml) of tap water, a clean tea spoon, any type of cold alcohol (stronger is better – vodka or gin), and cheek cells or any type of saliva substance. Firstly, start rinsing with the saline solution for 30 second, this is to extract the cells within the mouth and also cells around the cheeks. Then continue to spit the saline solution into a mix of 5ml of detergent and 15ml of tap water, thus hopefully transferring you DNA to the solution. With mechanical agitation for the duration of 5 minutes, within this time frame the tissue of the cheek cell should begin to burst and worsen the cell membrane and things surrounding the nuclei, which would lead into releasing the DNA within the cell. Next, you can continue to slowly pour your cold alcohol into the mixture, because DNA is insoluble in cold alcohol, the DNA would slowly appear into white strains. With the cold alcohol, other compounds would dissolve and the DNA would slowly be separated from the other compounds and would be visible to the naked
My hypothesis predicts that if we were to use improper or non-lab chemicals, the result DNA would become faulty and would result in an inadequate samples for further studies, it would seem that if we were to extract DNA without chemicals like lysis solution, concentrated salt solution, resuspenstion buffer, ethanol or isopropyl alcohol, it would result in poor DNA results.
Method one
Method one to create a perfect result and to extract the optimal DNA, I would believe that we would need centrifuge, sample tube, micropipettors, lysis solution, concentrated salt solution, resuspenstion buffer, …show more content…
We can get these cheek cells by just brushing the side of our mouth with a slab, but before we get the cells, we must rinse for 30 seconds to remove all the extra food or any other unwanted subjects. After we rinse then brushed the insides of the mouth to get the cheek cells, we can cut the end of the slab and put it into a sample tube. The first step into extracting the DNA is to mix the slab with some lysis solution, using the micropipettor we can add the desired amount of lysis solution, after adding the solution using …show more content…
Such as a saline solution (salted water), a clean glass, 1 tea spoon (5ml) of detergent, 3 tea spoon (15ml) of tap water, a clean tea spoon, any type of cold alcohol (stronger is better – vodka or gin), and cheek cells or any type of saliva substance. Firstly, start rinsing with the saline solution for 30 second, this is to extract the cells within the mouth and also cells around the cheeks. Then continue to spit the saline solution into a mix of 5ml of detergent and 15ml of tap water, thus hopefully transferring you DNA to the solution. With mechanical agitation for the duration of 5 minutes, within this time frame the tissue of the cheek cell should begin to burst and worsen the cell membrane and things surrounding the nuclei, which would lead into releasing the DNA within the cell. Next, you can continue to slowly pour your cold alcohol into the mixture, because DNA is insoluble in cold alcohol, the DNA would slowly appear into white strains. With the cold alcohol, other compounds would dissolve and the DNA would slowly be separated from the other compounds and would be visible to the naked