A mass quantity and variation of organisms live in different regions around the world. Each organism is classified into groups depending on the characteristics of the species, this is called taxonomy. Invented by the Swedish scientist, Carolus Linnaes, the taxonomy system simplifies organism classification by ordering the species into seven major categories: Kingdom, Phylum , Class , Order , Family , Genus , and Species (Meier, Shiyang, Vaidya, & Ng, 2006). However, what if the species is unknown and we cannot classify such species into a certain category? A practice to identify the species is by DNA barcoding.
DNA barcoding follows the principles of basic taxonomic practice of associating a name with a specific reference collection in conjunction with a functional understanding of species concepts (Kress, Wurdack, Zimmer, Weigt, & Janzen, 2005). Furthermore, DNA barcoding is designed to provide rapid, detailed and precise species identifications, using a very short genetic sequence from a standard part of the genome, being capable of distinguishing one species from another (Hebert & Gregory, 2005). A great analogy …show more content…
Using the Qiagen’s DNeasy Plant Mini Kit procedures, the experiment conducts DNA extraction to retain a short genetic sequence from the genome. Procedures such as PCR (Polymerase Chain Reaction) will be done to amplify and analyze the genome of the unknown, following the RBCL PCR Protocol (plant) in amplification of the genome. The purpose of a DNA barcoding experiment is to help collect and analyze sequenced data from the organism (plant or animal), use the extracted DNA to identify the species, and categorize the species, finding a relation between other organisms. Therefore, if an unknown sample has its DNA extracted following compatible protocols, then a matching sequence would appear, indicating identification when analyzing on a
DNA barcoding follows the principles of basic taxonomic practice of associating a name with a specific reference collection in conjunction with a functional understanding of species concepts (Kress, Wurdack, Zimmer, Weigt, & Janzen, 2005). Furthermore, DNA barcoding is designed to provide rapid, detailed and precise species identifications, using a very short genetic sequence from a standard part of the genome, being capable of distinguishing one species from another (Hebert & Gregory, 2005). A great analogy …show more content…
Using the Qiagen’s DNeasy Plant Mini Kit procedures, the experiment conducts DNA extraction to retain a short genetic sequence from the genome. Procedures such as PCR (Polymerase Chain Reaction) will be done to amplify and analyze the genome of the unknown, following the RBCL PCR Protocol (plant) in amplification of the genome. The purpose of a DNA barcoding experiment is to help collect and analyze sequenced data from the organism (plant or animal), use the extracted DNA to identify the species, and categorize the species, finding a relation between other organisms. Therefore, if an unknown sample has its DNA extracted following compatible protocols, then a matching sequence would appear, indicating identification when analyzing on a