Discovering Unknown 41 The overall reasoning for this experiment is to figure out what the unknown bacteria is through a process of tests and research. There are several differential media and selective & differential medias used throughout my findings, and all were done using aseptic technique. Based on the results of my test I came to a conclusion that my unknown 41 is Kocuria rosea. In order to deferiencate my unknown from the possibilities I went by the dichotomous key provided. After I preformed a test I would circle my results until I came to my conclusion which was Kocuria rosea. The first test was the Glucose Oxidation- Fermentation Test which gave me a negative response in both the test tubes (with and without mineral oil), which
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rosea or M. luteus. I conduct a Nitrate test to differentiate between nitrate and nitrite, in which nitrite can be further metabolized. My bacteria had a positive reaction with the A and B reagents by turning red to indicate nitrites presence. Therefore, I go on to perform a Gelatin Hydrolysis test which was negative, but may have had unreliable results due to the bacteria not having enough time to grow. So I went ahead and preformed a Coagulase test to see if fibrinogen is present and forms a clot in which my unknown did not and was considered negative. At that point I knew my unknown, because all the outcomes I have circled led to Kocuria …show more content…
The first reported spotting of this pathogen was in a pediatric peritoneal dialysis patient. The pathogen was difficult to isolate due to the similarities to other common pathogens. When trying to detect Kocuria rosea the results were delayed for more than a week due to growing requirements. The bacterium grows best in temperatures 25-35 degrees Celsius and is strictly aerobic. The organism can also be found in soil, water, and on the skin. It has a smooth look and a pink color. It is identified in the lab by conventional test. The results are the same as what I received in lab with the addition on being gram-positive, nonmotile, lysozyme-sensitive, and a positive esculin hydrolysis. Kocuria rosea is sensitive to penicillin, erythromycin, vancomycin and
rosea or M. luteus. I conduct a Nitrate test to differentiate between nitrate and nitrite, in which nitrite can be further metabolized. My bacteria had a positive reaction with the A and B reagents by turning red to indicate nitrites presence. Therefore, I go on to perform a Gelatin Hydrolysis test which was negative, but may have had unreliable results due to the bacteria not having enough time to grow. So I went ahead and preformed a Coagulase test to see if fibrinogen is present and forms a clot in which my unknown did not and was considered negative. At that point I knew my unknown, because all the outcomes I have circled led to Kocuria …show more content…
The first reported spotting of this pathogen was in a pediatric peritoneal dialysis patient. The pathogen was difficult to isolate due to the similarities to other common pathogens. When trying to detect Kocuria rosea the results were delayed for more than a week due to growing requirements. The bacterium grows best in temperatures 25-35 degrees Celsius and is strictly aerobic. The organism can also be found in soil, water, and on the skin. It has a smooth look and a pink color. It is identified in the lab by conventional test. The results are the same as what I received in lab with the addition on being gram-positive, nonmotile, lysozyme-sensitive, and a positive esculin hydrolysis. Kocuria rosea is sensitive to penicillin, erythromycin, vancomycin and