Introduction
Fed batch and perfusion batch have been used broadly in both biopharmaceuticals for years, manufacturing many different products. The choice of using one process over the other has largely boiled down to what suits a company more in terms of what is suitable to them or their preference of one process over the other. Thus when choosing these systems it is the companies long term future that is considered rather than their short term.
Fed batch and perfusion batch are the 2 most commonly used processes for mammalian cell culture processes. This is especially true for glycosylated proteins required in large amounts (Chu L, Robinson DK. Industrial choices for protein production by large-scale cell culture. Curr Opin Biotechnol. 2001;12:180–187.) Figure 1. Fed batch: small volume of concentrated solution added
A fed batch culture (figure 1) is an operation in which there is a gradual addition of the necessary nutrients during the culture cycle in order to improve growth and productivity. This addition can be fed continuously or intermittently through one or multiple flow streams. The culture broth is collected normally at the end of a cycle entirely or partially (if the reminder can be reused to inoculate for the next repeated run) (Lim, Janice, et al. "An …show more content…
This addition can be fed continuously or intermittently through one or multiple flow streams. The culture broth is collected normally at the end of a cycle entirely or partially (if the reminder ca be reused to inoculate for the next repeated run) (Lim, Janice, et al. "An economic comparison of three cell culture techniques."BioPharm International 24.2 (2011): 54-60.). For large scale up, the fed batch process is an attractive choice due to its operational simplicity, however this operation typically involves a high start-up
Fed batch and perfusion batch have been used broadly in both biopharmaceuticals for years, manufacturing many different products. The choice of using one process over the other has largely boiled down to what suits a company more in terms of what is suitable to them or their preference of one process over the other. Thus when choosing these systems it is the companies long term future that is considered rather than their short term.
Fed batch and perfusion batch are the 2 most commonly used processes for mammalian cell culture processes. This is especially true for glycosylated proteins required in large amounts (Chu L, Robinson DK. Industrial choices for protein production by large-scale cell culture. Curr Opin Biotechnol. 2001;12:180–187.) Figure 1. Fed batch: small volume of concentrated solution added
A fed batch culture (figure 1) is an operation in which there is a gradual addition of the necessary nutrients during the culture cycle in order to improve growth and productivity. This addition can be fed continuously or intermittently through one or multiple flow streams. The culture broth is collected normally at the end of a cycle entirely or partially (if the reminder can be reused to inoculate for the next repeated run) (Lim, Janice, et al. "An …show more content…
This addition can be fed continuously or intermittently through one or multiple flow streams. The culture broth is collected normally at the end of a cycle entirely or partially (if the reminder ca be reused to inoculate for the next repeated run) (Lim, Janice, et al. "An economic comparison of three cell culture techniques."BioPharm International 24.2 (2011): 54-60.). For large scale up, the fed batch process is an attractive choice due to its operational simplicity, however this operation typically involves a high start-up