Results
Understanding the structure of the kidney
The kidney consists of two main regions the medulla region and the cortex (see Figure 12, 13, 14 and 15). These two regions contain about one million tiny filtering structures called nephrons. Each nephron consists of a very small filter (glomerulus) which is attached to a tubule. As blood is passed through the nephrons, fluids and waste products are filtered out. What can be seen in Figures 12, 13 and 14 is the glomeruli and the ducts.
Control Rats
Figure 16 represents EGF expression (brown staining) within a control rat. Relatively low levels of EGF expression can be seen mostly within the cortex of the kidney. EGF expression can be seen within the cytoplasm of the cells (A), whilst no …show more content…
Very high levels of EGF expression (lots of brown staining) can be seen within the cytoplasm of the tubules, but not in the glomeruli. The glomeruli have increased in size and tubules are dilated.
Diabetic rat treated with FT061
Figure 18 represents EGF expression within a Diabetic rat treated with FT061. EGF expression is extremely low and can only be seen in the cytoplasm of the tubules. Tubules are not dilated and glomeruli have not increased in size.
Overall EGF expression in experimental groups
Figure 19 represents the mean (and the range of standard error (±S.E)) percentage of EGF expression in the cortex of each kidney section per experimental group. By collating the data produced from aperio scan scope the amount of EGF expression in each rat was quantified. Control rats had a very small amount of EGF expression in the cortex of their kidneys (approximately 7%). The Diabetic rat kidney sections had a much higher expression of EGF around 13% of staining per kidney section; and diabetic rats treated with FT061 had the smallest amount of EGF expression per kidney section (approximately
Understanding the structure of the kidney
The kidney consists of two main regions the medulla region and the cortex (see Figure 12, 13, 14 and 15). These two regions contain about one million tiny filtering structures called nephrons. Each nephron consists of a very small filter (glomerulus) which is attached to a tubule. As blood is passed through the nephrons, fluids and waste products are filtered out. What can be seen in Figures 12, 13 and 14 is the glomeruli and the ducts.
Control Rats
Figure 16 represents EGF expression (brown staining) within a control rat. Relatively low levels of EGF expression can be seen mostly within the cortex of the kidney. EGF expression can be seen within the cytoplasm of the cells (A), whilst no …show more content…
Very high levels of EGF expression (lots of brown staining) can be seen within the cytoplasm of the tubules, but not in the glomeruli. The glomeruli have increased in size and tubules are dilated.
Diabetic rat treated with FT061
Figure 18 represents EGF expression within a Diabetic rat treated with FT061. EGF expression is extremely low and can only be seen in the cytoplasm of the tubules. Tubules are not dilated and glomeruli have not increased in size.
Overall EGF expression in experimental groups
Figure 19 represents the mean (and the range of standard error (±S.E)) percentage of EGF expression in the cortex of each kidney section per experimental group. By collating the data produced from aperio scan scope the amount of EGF expression in each rat was quantified. Control rats had a very small amount of EGF expression in the cortex of their kidneys (approximately 7%). The Diabetic rat kidney sections had a much higher expression of EGF around 13% of staining per kidney section; and diabetic rats treated with FT061 had the smallest amount of EGF expression per kidney section (approximately