ABSTRACT
Dex-Ibuprofen–polyethylene glycol (PEG) conjugates (PEG-dex-Ibu) were organized and their prospective as a prolonged release method was investigated. PEG-Dex-Ibuprofen conjugates were synthesized as of Ibuprofen and PEG with 4000 Da molecular weights by esterification in the incidence of DCC and DMAP. The PEG-dex-Ibu conjugates were characterized by FT-IR, 1H NMR, and DSC analysis. The solubility revise in aqueous system showed an improvement within the solubility of conjugates. The dissolution studies showed an accurate acid–base catalysis prototype reliant on the pH of the medium. This indicated a fine chemical constancy in aqueous buffer solution of acidic medium and the comprehensive discharge performance was found in prodrug …show more content…
The solubility was observed by sedimentation of particles.
Standard calibration curve of Dex-ibuprofen
Dex-ibuprofen (10 mg) was dissolved in 100 mL methanol. From these stock solution aliquots of 0.2,0.4,0.6,0.8,0.10 µ/mlwere withdrawn in 10 mL volumetric flask and diluted up to the volume with methanol and phosphate buffer (ph 7.4) solutions to give concentrations of 2,4,6,8,10 g/mL. Absorption of each solution as calculated at 227.2 nm ,223nm using Elico SL 210 UV-1700 UV Visible double beam spectrophotometer and methanol as well as phosphate buffer (ph 7.4) as a reference standard correspondingly. Compatibility studies
The compatibility of drug and polymers under experimental condition is significant precondition before formulation. Incompatibility between drugs and excipients can modify stability and bioavailability of drugs, thereby, moving its safety and efficacy. Study of drug–excipients compatibility is a vital process in the growth of a stable dosage form. Drug–excipients compatibility testing at an early stage helps in the assortment of excipients that increases the prospect of developing a stable dosage …show more content…
The mixture was stirred, and a white precipitate of dicyclohexyl urea (DCU) was created after 15–30 min. The reaction was monitored by thin layer chromatography (TLC). After approximately 6–8 h, the precipitate was filtered and the filtrate was evaporated to dryness. The resulting residue was dissolved in acetone, filtered to additional remove any DCU, and evaporated once more. 10% sodium bicarbonate was used to separate the PEG–dex-Ibu from free dex-Ibuprofen. The purified products were dried under vacuum. The pure PEG4000–dex-Ibu conjugate with the yield of 60.49% was obtained. The fact that there was no free drug of Dex- Ibuprofen available in polymer prodrug was established by thin layer chromatography and DSC measurements [25]. The scheme was mentioned in Figure no
Dex-Ibuprofen–polyethylene glycol (PEG) conjugates (PEG-dex-Ibu) were organized and their prospective as a prolonged release method was investigated. PEG-Dex-Ibuprofen conjugates were synthesized as of Ibuprofen and PEG with 4000 Da molecular weights by esterification in the incidence of DCC and DMAP. The PEG-dex-Ibu conjugates were characterized by FT-IR, 1H NMR, and DSC analysis. The solubility revise in aqueous system showed an improvement within the solubility of conjugates. The dissolution studies showed an accurate acid–base catalysis prototype reliant on the pH of the medium. This indicated a fine chemical constancy in aqueous buffer solution of acidic medium and the comprehensive discharge performance was found in prodrug …show more content…
The solubility was observed by sedimentation of particles.
Standard calibration curve of Dex-ibuprofen
Dex-ibuprofen (10 mg) was dissolved in 100 mL methanol. From these stock solution aliquots of 0.2,0.4,0.6,0.8,0.10 µ/mlwere withdrawn in 10 mL volumetric flask and diluted up to the volume with methanol and phosphate buffer (ph 7.4) solutions to give concentrations of 2,4,6,8,10 g/mL. Absorption of each solution as calculated at 227.2 nm ,223nm using Elico SL 210 UV-1700 UV Visible double beam spectrophotometer and methanol as well as phosphate buffer (ph 7.4) as a reference standard correspondingly. Compatibility studies
The compatibility of drug and polymers under experimental condition is significant precondition before formulation. Incompatibility between drugs and excipients can modify stability and bioavailability of drugs, thereby, moving its safety and efficacy. Study of drug–excipients compatibility is a vital process in the growth of a stable dosage form. Drug–excipients compatibility testing at an early stage helps in the assortment of excipients that increases the prospect of developing a stable dosage …show more content…
The mixture was stirred, and a white precipitate of dicyclohexyl urea (DCU) was created after 15–30 min. The reaction was monitored by thin layer chromatography (TLC). After approximately 6–8 h, the precipitate was filtered and the filtrate was evaporated to dryness. The resulting residue was dissolved in acetone, filtered to additional remove any DCU, and evaporated once more. 10% sodium bicarbonate was used to separate the PEG–dex-Ibu from free dex-Ibuprofen. The purified products were dried under vacuum. The pure PEG4000–dex-Ibu conjugate with the yield of 60.49% was obtained. The fact that there was no free drug of Dex- Ibuprofen available in polymer prodrug was established by thin layer chromatography and DSC measurements [25]. The scheme was mentioned in Figure no