Optimum Temperature Of Amylase

Improved Essays
1. Our experiment supported our hypothesis. The optimal temperature for the activity of the human amylase is 37°C. This suggest that enzymes have to adapt to the temperature of the organisms in order to develop work because the normal temperature of human is 37°C. It is important to understand that the enzyme amylase play a role to breakdown, so other enzymes can produce sugar of it.
In humans, it is critical to develop work at human’s temperature to continue developing break down the molecules. For example, it is important in humans, so it can break down all the starch by hydrolysis. This helps the human to make digestion. Many food has a big quantity of starch and a small amount of sugar.
According to the bioinformatics study, the sequence
…show more content…
According to our hypothesis, the optimal temperature of the three different amylase will be different. Thus, it supported our hypothesis. The variation among the three different amylase is depend on the environment where they can be found. We can determine by this information that in fact enzymes have to adapt to their environment to help the systems and organisms to …show more content…
The experiment about pH brace our hypothesis because the pH of the enzyme amylase is 7. It has correlation because human beings have pH of 7 as well. The pH of 7 is known as neutral. This means that the concentration of H+(hydrogen) ions and OH- (hydroxide) ions is 10⁻⁷.
For example, saliva is an enzyme amylase in the human body. It needs to have a pH of 7 will not denature because it is neutral. Therefore, the amylase will be effective. Also, it has adapted to the pH, so it can breakdown the food that human beings inherit to make it useful for other enzymes.
By using the bioinformatics information, we can determine that the non polar amino acids affect the bonding with hydrogen bonds. This means that the pH of the enzyme will be physiological. It can work better at a pH concentration of 7, and actually the cells can survive in that environment. Meaning, enzymes can work well with this concentration of pH, so it bonds do not break easily when it can be saturated of acid or base.

4. As our experiment says, the three different amylase will have different optimal pH concentration. However, the results show a difference and a similarity. The fungi amylase works the best at a pH of 5, which it makes it logical because fungi are found in soil areas in which the pH is more

Related Documents

  • Great Essays

    Amylase Experiment

    • 1123 Words
    • 5 Pages

    By adding more temperatures, the results become more precise as to what temperature amylase denatures at, and what temperature is the most efficient for amylase. Variations that differ from the variables in this experiment could be the effect of pH levels on enzyme activity, which would show the best pH levels for amylase to be most efficient and whether it works best at low, medium or high pH levels. The concentration of amylase would also be a variation that would show whether amylase works faster if there is more of it or not. If all of these variations were done, it would show in what conditions amylase works best at, and if the human body fits those conditions or…

    • 1123 Words
    • 5 Pages
    Great Essays
  • Improved Essays

    its was higher at 8.5 3. State the optimum temperature for sucrase activity and describe how sucrase activity changes at lower and higher temperatures. Table 3: Effect of Sucrose Concentration on Sucrase Activity Optical Density 35 g/L 30 g/L 25 g/L 20 g/L 15 g/L 10 g/L 5 g/L 0 g/L 1 0.984 1.011 0.948 0.894 0.821 0.773 0.500 0.002 2 0.985 0.995 0.945 0.931 0.821 0.772 0.501 0.003 3 0.981 1.005 0.945 0.924 0.826 0.736 0.524 0.001 average 0.983 1.000 0.946 0.916 0.823 0.760 0.508 0.002 Laboratory Report/ Angela Collins/ Enzyme Activity/ Catherine Rice/ 07.09.2014/ Page [2] of…

    • 1040 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Lowest? The highest was at pH 7, and the lowest was at pH 2. The catalase enzyme works the best at an optimal pH of 7 (neutral) and works the worst at an acidic pH of 2. How does changing the pH affect the rate of enzyme activity? Does this follow a pattern you anticipated?…

    • 680 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Jello-O Enzyme Lab

    • 581 Words
    • 3 Pages

    Enzymes are protein molecules that significantly speed up the rate of virtually all of the chemical reactions that take place within the cell. In the following lab was to examine an enzyme that is found in pineapples. That enzyme is bromelain its breaks down protein into their protein into their amino acid by a process of hydrolysis. They are found in tropical areas like Hawaii, Thailand, and Brazil. For the first experiment the hypothesis was that the when gelatin was added to the water, fresh pineapple juice, and canned pineapple juice and submerged into the ice chest the solutions would turn into Jell-O.…

    • 581 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Catalase Experiment

    • 881 Words
    • 4 Pages

    The effect of altering temperature on the rate of liver enzyme activity is measured in this experiment to be able to visualize how temperature in the environment and in the body can manipulate the rate of enzyme activity. Enzymes are protein catalysts that lower the amount of activation energy required to start a biochemical reaction. According to Dr. Meyertholen (2015), enzymes are structured to work with certain substrates, meaning that the structure determines the function of the enzyme. If the enzymes shape is altered, they become denatured. Denaturation of an enzyme can occur with changes in factors such as temperature, pH, or salinity.…

    • 881 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Liver Lab

    • 544 Words
    • 3 Pages

    The difference between the two graphs is the speed of the reaction. The temperatures of the two tests were very similar throughout the experiment. when doing this experiment one should have a paper towel ready to wipe the side of the test tube to be able to read the temperature. Conclusion: This experiment tested the difference between a untreated and treated catalase enzyme.…

    • 544 Words
    • 3 Pages
    Improved Essays
  • Decent Essays

    In most cases, a chemical reaction is able to occur in more than one way; enzyme kinetics studies the effect of how biological catalysts can influence biochemical reactions. Enzymes play an important role in regulating metabolic pathways in the human body and without enzymes they will not function efficiently. The rate of a reaction is enhanced by enzyme activity which provides alternate pathways for the reaction to proceed by.…

    • 177 Words
    • 1 Pages
    Decent Essays
  • Superior Essays

    Amylase Biology Lab Report

    • 2820 Words
    • 12 Pages

    Considering there was no temperature in trials between these two temperatures, it was determined that the optimal temperature for bacterial amylase ranges between 40° C and 55° C. Furthermore, Table 1.1 demonstrates no apparent enzyme function at 80° C, considering there was no color shade change as determined by the color shade values. Table 1.2 illustrates results that are alike for fungal amylase. Similar to bacterial amylase, the highest enzyme activity appeared to be at 55° C, considering the color shade value at that specific temperature was the lowest. Furthermore, unlike the bacterial amylase, the color values in table 1.2 demonstrate that the enzyme was functioning slightly at 80° C because there was a slight color…

    • 2820 Words
    • 12 Pages
    Superior Essays
  • Improved Essays

    The temperature is too low inside many living things, which makes it hard for biochemical reactions to happen at a faster rate. Enzymes help biochemical reactions speed up. An enzyme works by lowering the amount of activation energy needed to start a chemical reaction. Activation energy is the minimum amount of energy needed for the reactants to begin a chemical reaction. Normally, biochemical reactions would take many days, but with enzymes doing their job correctly, the same reaction is able to happen in seconds.…

    • 811 Words
    • 4 Pages
    Improved Essays
  • Superior Essays

    However, this was not the case in the experiment, meaning this part of the hypothesis is rejected. In fact, at a pH of 7, the enzyme activity has already begun to decrease. Rather, the optimal pH for enzyme activity was at a pH between 5 and 6. The first part of the hypothesis (that is pH affects enzymes) fails to reject because pH did affect enzyme…

    • 1717 Words
    • 7 Pages
    Superior Essays
  • Improved Essays

    The purpose of conducting this experiment was to explore how different factors affect the reaction rate of enzymes reacting with their corresponding substrates in order to learn more about how enzymes function in different environments. The independent variables investigated in this experiment were the concentration of different substrates, the temperature of the environment, and the effect of a catalyst on the reaction rate. The dependent variable for all of the investigations was the time it took for the reaction to occur. To investigate the effect of the concentration of the substrate on the reaction time, four test tubes were used.…

    • 1027 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    However, when the substrate concentration was increased, the intensity of color stopped increasing due to the enzymes being unable to to process at a faster rate. All of the alternate hypothesizes were supported; in the experiments, section one (with Figure 1) had an optimal temperature at approximately 24°C; section two (with Figure 2) had an optimal pH at approximately pH8; in section 3 (with Figure 3) the color continued to increase with the addition of more enzymes; and in section 4 (with Figure 4) the color continued to increase until it reached a maximum velocity with the addition of more substrates. One desired retrial for the experiment would be to test for more points after 20 drops of potato juice in Figure 3. Understanding the effects of how these properties alter the ability of the catecholase enzyme could allow for better insight into decreasing time needed for biochemical reactions, proper food storage, and the condition of…

    • 753 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    The experiment conducted was to observe the enzyme catalysis of Horseradish Peroxidase with different determining factors. In this experiment the horseradish peroxidase enzyme should have an optimal temperature of 35°C, should have an optimal pH of 6, and should catalyze rapidly with the substrate and enzyme concentration. We were able to observe that the enzyme reacts best with its optimal temperature of 35° C and optimal pH of 6. Moreover, when there’s a high the substrate concentration and a high enzyme concentration we can observe a rapid enzyme catalysis. The hypothesis was partially falsified for the optimal pH and the optimal temperature were both different than that stated in the hypothesis.…

    • 1134 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    The purpose of this experiment is to conduct and test the effects of catechol oxidase with varying temperatures. Based on many scientific journals, changes on different enzymes are affected by temperature (Pao-Huei et al. 2016). These concluded that the reaction of catechol oxidase is accelerated…

    • 1142 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Starch Lab Report

    • 1016 Words
    • 5 Pages

    The presence of reducing sugars led to colour change in the test tube with amylase solution. An error that may have occurred in this experiment was the amount of time that the test tubes were kept in the water bath. The manual stated to only keep the hydrolyzed solution incubated for 10 minutes but never stated how long to keep the reaction part of the experiment in the water bath for. We kept the test tubes in boiling water bath for about 15 minutes and came out with only reaction with our amylase solution. If we kept the test tubes in the water bath for a little longer the saliva may have reacted and changed colour too.…

    • 1016 Words
    • 5 Pages
    Improved Essays