The results of the experiment supported my hypothesis, which stated that with a decrease in the pH of blood, there should be an increase in the release of oxygen by haemoglobin. The oxygen dissociation curve (ODC), which was created, using the data collected in the experiment, demonstrates that the hypothesis was accurate.
In a similar experiment titled, “Continuous Determination of the Oxygen Dissociation Curve for Whole Blood”, Rossi-Dernardi et al. (1975) found that the pH of the blood used and the partial pressure of oxygen had an effect on the affinity of oxygen in haemoglobin. The shape of the ODC in the article is very similar to Figure 1. This illustrates that the results of my experiment were correct. Some possible errors, which could have occurred, were letting the blood re-oxygenate slightly, changing the oxygen pressure within the tubes. This could have potentially …show more content…
In Zijlstra, et al. (1991) experiment the results showed that there were differences between the different types of blood used due to the diverse haemoglobin and the partial pressure of oxygen in each. As in my experiment the different of pH levels had dissimilar light absorptions and oxygen binding affinities. As the pH was lowered, the affinity for oxygen increased, matching this experiment.
The Bohr effect shows the dependence of oxygen affinity on the pH of the blood. The body benefits from this effect when exercising due to an increase in respiratory rate, and the need for oxygen. While exercising your body needs to release carbon dioxide and other wastes faster, in exchange for oxygen. This Bohr effect justifies that with an increase of waste concentration, haemoglobin has a tendency to release more oxygen molecules more quickly (LeBouef-Little, 2013). This in return is very important to the body and benefits it
In a similar experiment titled, “Continuous Determination of the Oxygen Dissociation Curve for Whole Blood”, Rossi-Dernardi et al. (1975) found that the pH of the blood used and the partial pressure of oxygen had an effect on the affinity of oxygen in haemoglobin. The shape of the ODC in the article is very similar to Figure 1. This illustrates that the results of my experiment were correct. Some possible errors, which could have occurred, were letting the blood re-oxygenate slightly, changing the oxygen pressure within the tubes. This could have potentially …show more content…
In Zijlstra, et al. (1991) experiment the results showed that there were differences between the different types of blood used due to the diverse haemoglobin and the partial pressure of oxygen in each. As in my experiment the different of pH levels had dissimilar light absorptions and oxygen binding affinities. As the pH was lowered, the affinity for oxygen increased, matching this experiment.
The Bohr effect shows the dependence of oxygen affinity on the pH of the blood. The body benefits from this effect when exercising due to an increase in respiratory rate, and the need for oxygen. While exercising your body needs to release carbon dioxide and other wastes faster, in exchange for oxygen. This Bohr effect justifies that with an increase of waste concentration, haemoglobin has a tendency to release more oxygen molecules more quickly (LeBouef-Little, 2013). This in return is very important to the body and benefits it