How To Determine The Concentration Of Carbohydrates

Superior Essays
Practical Report
(Determination of blood glucose levels and qualitative carbohydrate tests)

Introduction:
The term ‘’Carbohydrates’’ is defined as the carbon compounds which contains hydroxyl groups in large quantities (King, 2013). Carbohydrates are those organic compounds that have the empirical formula CnH2nOn, or (CH2O)n (Fromm 1997). Carbohydrates are one of the four types of organic compounds in living cells that are produces during photosynthesis and are the important source of energy in both plants and animals (Ahmed 2014). They are built from monosaccharides. These monosaccharides are the small molecule that consists of three to nine carbon atoms and differ in size and in the s configuration at
…show more content…
As we got the absorbance values for the three unknown solutions and the equation from the scatter graph y=0.045x + 0.0791,we did calculations as follows.
Concentration of Unknown A, y=0.410 y=0.0467x + 0.0424
0.0467x = 0.410 - 0.0424 = 0.3676 x = 0.3676/0.0467 = 7.8715 = 7.87
Concentration of Unknown B, y = 0.240 y = 0.0467x + 0.0424
0.045x = 0.240 – 0.0791 = 0.1976 x = 0.1976/0.0467 = 4.2312 = 4.23
Concentration of Unknown C, y= 0.698 y = 0.0467x + 0.0424
0.0467x = 0.698 – 0.0424 = 0.6556 x = 0.6556/0.0467 = 14.038 =
…show more content…
We noticed positive response for all the four solutions A, B, C and D as the solution was in purple colour in all the test tubes. We started our second qualitative test which is an iodine test by taking mixed carbohydrate solution in 2ml pipette in to four test tubes A, B, C and D and 3 drops of dilute iodine solution was added immediately to the four test tubes where we have seen negative reactions to the three solutions A, B, C as the solution was in yellow colour and a positive reaction to the solution D as the solution turned to blue-black colour. Later we did the benedict’s test by adding benedict solute (CuSO4) in a 2.5ml pipette and mixed with 1ml mixed carbohydrate solution. The test tube was kept on a stand in a boiling water bath for 2mins and was removed to check the reactions. We have noticed positive reactions to solutions A and B whereas a negative reaction to solutions C and D as the solution is red colour. Finally we did the seliwanoff’s test by adding 2.5ml of seliwanoff’s reagent (orcinol) with 2.5ml pipette in to all the four test tube and then we also added 5 drops of mixed carbohydrate solution. Later we have kept the test tubes on stand in a boiling water bath for 10 minutes

Related Documents

  • Improved Essays

    Demonstration of Cellular Respiration Inside Lima Beans Cells in Vitro Purpose: Cellular respiration is a series of metabolic reactions that take place in the mitochondria and produce chemical energy in the form of ATP by the breakdown of food molecules. There are three processes involved in cellular respiration; glycolysis, the Krebs cycle and the electron transport chain. During this processes, glucose is oxidized by a series of redox reactions and its electrons and hydrogen ions are donated to two electron carriers called NAD+ and FAD.…

    • 776 Words
    • 4 Pages
    Improved Essays
  • Decent Essays

    Monosaccharides are glucose and fructose, while disaccharides are sucrose and lactose. These function to store energy in the body. Macromolecules…

    • 291 Words
    • 2 Pages
    Decent Essays
  • Superior Essays

    MR test tube inoculated and incubated for 24 hours, ten to fifteen drops of methyl red indicator then added to the cultured. If it turned red then that was the positive for the presence of mixed acids. No color change was negative, however, if the media turned orange, it was inconclusive. VP test tube was inoculate and incubated up to 3 days, vortexed, added Barrits Reagent A (alpha-napthol) and Barrits Reagent B (potassium hydroxide). Red or pink color was positive reaction, no color change was negative, however it could take up to an hour to see the color change.…

    • 1514 Words
    • 7 Pages
    Superior Essays
  • Decent Essays

    Chem 115 Lab Summary

    • 246 Words
    • 1 Pages

    To begin Lab 10 of Chem 115, students received permission to start the lab procedure after completion of prelab. First, students made a 25.0 mL of a 0.15 M Malonic Acid and 0.20 M Manganese Sulfate Monohydrate solution and labeled it "Solution 1". After the solution was made, a piece of parafilm was used to seal the flask before mixing it well. Next, a 25.0 mL of a 0.20 M Potassium Iodate solution, using 0.080 M sulfuric acid as a solvent, was made. Before adding the acid, students heated about 25 mL of the acid in a 100 mL volumetric flask.…

    • 246 Words
    • 1 Pages
    Decent Essays
  • Improved Essays

    Testing for the cation ammonium used two 10mL graduated cylinders and a 50mL burette. Added 1mL of 6M NaOH to 1mL of unknown solution created in the solubility test. Smelled vapor and recorded any smell of ammonia. Testing for halides involved using three 10mL graduated cylinder and one 50mL burette. Added 1mL of 6M HNO_3 and 1mL of 0.1M AgNO_3 to 1mL of solution created from solubility test and recorded any white precipitates.…

    • 1965 Words
    • 8 Pages
    Improved Essays
  • Improved Essays

    Cations Lab

    • 929 Words
    • 4 Pages

    You should have 10 clear test tubes, bath them by hot water. Test 1a: put a known solution in tube 1 and add three drops of each of the 10 cation solutions. Add 8 drops 6 M HCl solution to tube 1 and do it one more time. If it forms precipitate, you should continue add HCl. Record this data on table 1a.…

    • 929 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Monosaccharides are the unmistakable sugars. Two monosaccharides make a disaccharide. Three or more monosaccharides are a polysaccharide. Glucose, fructose, and galactose are monosaccharides. Sucrose, lactose, and maltose are disaccharides.…

    • 509 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Carbs Research Paper

    • 574 Words
    • 3 Pages

    Carbs contain the elements of carbon, hydrogen, and oxygen, and like lipids always have a hydrogen, oxygen ratio of 2:1. Carbs typically have a shape of a hexagon/circle thing. The body cannot digest all carbohydrates in the diet, however; indigestible carbohydrates, known as fiber, travel through the intestines and can help maintain proper digestive health. Carbohydrates are known for short-term energy storage. Functions of Lipids…

    • 574 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Macromolecule Lab Report

    • 708 Words
    • 3 Pages

    A macromolecule is a molecule that contains any "large number" of atoms. There are four main different categories of macromolecules that make up our body: carbohydrates, lipids, proteins, and nucleic acids. (1) Carbohydrates are sugars that store and transport energy. (2) Lipids also store energy and also are signalling molecules. (3) Proteins are linear chains of amino acids that help with the structure, function, and regulation of the body’s tissues and organs.…

    • 708 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Macromolecules are large molecules that are composed of smaller units. The four major macromolecules are carbohydrates, lipids, nucleic acids, and proteins. Carbon is the primary component of the four macromolecules. Carbon's molecular structure allows it to bond in many different ways and with many different elements. A Monomer is an atom or small molecule that may bind chemically to other monomers to form a Polymer Different monomers make up a polymer.…

    • 987 Words
    • 4 Pages
    Improved Essays
  • Superior Essays

    Introduction What are enzymes and how do they affect reactions? Enzymes force reactions, in cells, to maintain a speed necessary for life. Enzymes can also be considered catalysts because they require the reactions to arise faster without themselves changing. For this experiment, the substrate in the enzymatic reaction was sucrase, and it will be causing sucrose to change resulting in the following equation:…

    • 1790 Words
    • 8 Pages
    Superior Essays
  • Great Essays

    BIOLOGY LAB REPORT. ENZYME ACTIVITY Planning (a) – Pl (a): Research Question: How does the concentration of the salivary amylase affect its activity? Aim:…

    • 768 Words
    • 4 Pages
    Great Essays
  • Great Essays

    Purpose The purpose of this experiment is to find the pKIn blue using spectrophotometry. Bromophenol blue dissociate in water by the following reaction: i) HIn + H2O ⇋ H3O+ + In- The KIn value can be calculated from the reaction: ii) K_In=[H3O+]…

    • 1630 Words
    • 7 Pages
    Great Essays
  • Improved Essays

    Carbohydrates Essay

    • 777 Words
    • 4 Pages

    Carbohydrates can be classified into three groups: monosaccharide, disaccharides and polysaccharides. Monosaccharide is simple sugars, and its main representatives are glucose (manufactured by plants during photosynthesis), fructose (found in honey) and lactose (found in milk). The union of two monosaccharaides forms disaccharides, and his best-known representatives are sucrose and lactose. Sucrose consists of a glucose molecule and a fructose molecule. Sucrose is the sugar we consume at home, which we use in our coffee, juices, pastries etc.…

    • 777 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Lab Report Cation Essay

    • 875 Words
    • 4 Pages

    CH 204- Introduction to Chemical Practices Experiment 1- Qualitative Analysis of Cations Sharon Alexander Kathrynn Labat TA: Yu-Chun Lin September 25, 2017 Results & Discussion This experiment was composed of three parts. In the first part, qualitative methods were used to identify the unknown cation in an aqueous solution.…

    • 875 Words
    • 4 Pages
    Improved Essays