Dengue Fever Case Study

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Register to read the introduction… Symptoms usually occur from three to fifteen days after exposure to an infected exposure.

Dengue Fever: The symptoms of Dengue Fever are characterized by an abrupt fever lasting for 5-7 days, severe headaches, muscle and joint pains, and rash. The clinical features may vary according to the age of the patient.

Dengue Hemorrhagic Fever: The hemorrhagic form of dengue fever induce more severe symptoms. These effects include: loss of appetite, vomiting, high fever, abdominal pain, and intense headaches. Circulatory failure and shock may occur in severe cases. If the disease is left untreated, the disease results in death of over half of individuals who are infected.


Currently there is no cure and no specific treatment available, however, lives have been saved under clinical observation and care. Vaccine development for Dengue and Dengue Hemorrhagic Fever is difficult because any of the four dengue viruses may cause disease. An immunization of only one or two of the viruses could actually inflate the risk of serious disease. Progress is being made to solve this problem.

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It was first determined that it would be possible to use a modified avian retrovirus (dsSIN) to insert specific DNA sequences into certain mosquito cells. The cells infected in the salivary glands of the mosquito by the avian virus were the same cells of the salivary gland infected by the DEN virus. This meant that it would be possible to program antisense RNA strands to crucial RNA coding strands of the DEN virus. 2. A dsSIN virus was modified to include a 567-base antisense RNA that was targeted to disrupt a premembrane protein in the DEN-2 (DEN-2 is one of the 4 types of DEN virus mutations.) viral genome. Inhibition of the transcription of this protein prevented the formation of the virus. 3. The exact sequence of the 567 bases was determined by sequencing the promoter region of the viral genome. The complementary RNA bases to this sequence determined the code of the antisense RNA strand. 4. To determine whether they had inserted the 567-bases, researchers performed a Northern blot analysis on a control of the avian virus and of the target cells of the virus. The target cells that contained the antisense RNA coding were 567 base pairs longer, and thus could be differentiatied by their location on the agarose

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