Therefore, evaluation of negative impacts of pollutants on living organisms in the form of toxicity testing is routinely used to produce baseline data for environmental hazard and risk assessment of chemicals.94 Daphnia magna, an important link in the food chain of aquatic ecosystem, which is highly sensitive to toxic substances has been used to understand the toxic effect of waste effluent.94 To date, some explicit studies to understand effects of multiple stressors on aquatic ecosystem have been reported that are hints of how mange the freshwater in the wake of multiple stressors.95–98 The current project helps understand and compare toxicity of different stage of wastewater from influent to effluent in their intact and unaltered form.
