Figure 22. Relative mRNA expression in INS-1E cells after different chemicals treatment. Cells were treated with DHT, TCS and TCC for 48 h before RNA extraction for activity determination. Expression levels of the AR, pdx1, GK and Ins2 were determined by RT-QPCR and mRNA expression levels were normalized with TFIIB (A-D). DMSO treated samples were considered as control. Control samples were set as one and results are shown as mean ± S.E.M of duplicate samples from three independent experiments. Significant levels were determined using students unpaired T-test.
Discussion
Daphnia Magna
The bioaccumulation and persistency of TCS and TCC in the environment can affect various organisms, especially algae, aquatic resources and human. Recently, their endocrine disrupting capabilities have become major concern to humans; especially with their regular exposures to various prod¬ucts which have direct contact with the body. This study investigated the endocrine disrupting effects of xenobiotics TCS and TCC both in vivo and in vitro; as well as in single celled organisms, invertebrates and vertebrates. Toxic effects of TCS and TCC were investigated in invertebrate model (D. magna), yeast cell, human pancreatic beta cell; …show more content…
According to this category both TCS and TCC are highly toxic to aquatic organisms[97, 98]. Data from this study demonstrated that 48h LC50 of TCS and TCC on D. magna were 1.354µM (0.39mg/L) and 0.11µM (0.034mg/L). Comparing the toxic effect of TCS and TCC, TCC is more toxic than TCS on D. magna which is very much similar to other study report[99] . Ecotoxicity data obtained for TCS and TCC in the present study are mostly comparable to those obtained by other researchers (and organizations)
Discussion
Daphnia Magna
The bioaccumulation and persistency of TCS and TCC in the environment can affect various organisms, especially algae, aquatic resources and human. Recently, their endocrine disrupting capabilities have become major concern to humans; especially with their regular exposures to various prod¬ucts which have direct contact with the body. This study investigated the endocrine disrupting effects of xenobiotics TCS and TCC both in vivo and in vitro; as well as in single celled organisms, invertebrates and vertebrates. Toxic effects of TCS and TCC were investigated in invertebrate model (D. magna), yeast cell, human pancreatic beta cell; …show more content…
According to this category both TCS and TCC are highly toxic to aquatic organisms[97, 98]. Data from this study demonstrated that 48h LC50 of TCS and TCC on D. magna were 1.354µM (0.39mg/L) and 0.11µM (0.034mg/L). Comparing the toxic effect of TCS and TCC, TCC is more toxic than TCS on D. magna which is very much similar to other study report[99] . Ecotoxicity data obtained for TCS and TCC in the present study are mostly comparable to those obtained by other researchers (and organizations)