The knife that was used during the murder contained Ms. Knox's DNA on the handle, and a small amount of murdered victim Kercher's DNA on the blade. (Brayson, 2016) The murder of Ms. Kercher took place in her bedroom whereas the bra clasp belonging to the victim found contained traces of Sollecito's DNA. Another individual involved Rudy Guede’s DNA was found throughout Ms. Kercher's bedroom and mixed with her blood in a bag from a vaginal swab taken from Ms. Kercher, and also Guede’s bloody handprints and fingerprints covered the bedroom where Ms. Kercher was murdered. (Brayson, 2016)…
The advancement of forensic science has progressed immensely since its conception. The contributions it has made for the legal system are immense. One such example are its techniques used to extract DNA during forensic cases. DNA is considered to be one of the most well regarded and highly assessed sources of information (Gershaw et al., 2010). Another important development used during investigations is CODIS or the Combined DNA Index System.…
1. Do you think that Tyrone Briggs might be a victim of false identification? Why or why not? I think that Tyrone Briggs might be a victim of false identification for two reasons.…
To compare a specimen using Gel electrophoresis, you must first take the DNA of a bald eagle and your specimen and separate the mixtures of DNA, RNA, or proteins according to molecular size. In gel electrophoresis, the molecules must be separated and pushed by an electrical field through a gel that contains microscopic pores. The molecules travel through the tiny pores in the gel at a fast speed according to there length. This means that a small DNA molecule will travel a greater distance through the gel than will a larger DNA molecule. Next, you need to find the patterns that link the two DNA samples similarities.…
My phage has the wonderful name of Lorge. This name is very special because it offers information about the phage right away. I also chose this name because the first word that Tracy said when she saw my phage was, “Wow, it’s huge!” To get this wonderful phage I collected the soil sample from the flower pots across the street outside of Bessey Hall. I got the sample at the start of class on September 11, 2017.…
PCR clean-up was tested using the same electrophoresis method as the DNA extraction. PCR A portion of DNA from the extraction was amplified using polymerase chain reaction (PCR). A new sterile tube was used. In the tube 23.0µL of master mix was added which contained 10.5µL of water, 12.5µL Green Taq( premix with buffer, Taq, and dNTPs).…
The first step in this procedure is to prepare a buffer containing 1% of the liquid agarose gel. Once the gel solidifies within 15 to 20 minutes, a mold should have formed. Next, a comb is inserted into the chamber to seperate the gel creating tiny holes called “wells”. The comb is removed carefully so it will not disrupt the wells inside the chamber. The DNA prepared can be loaded into the buffer using a micropipette.…
With time and budget constraint putting more pressure on the Arizona Game and Fish Department to produce more reports with less resources. Environmental DNA sampling could be the answer to acquiring population data, faster than traditional sampling methods. Surveying populations of animals, to observe the status of their populations has been a difficult task for Wildlife Biologists, since the late 1800s. The recent decline of wildlife populations has put more pressure on wildlife managers to determine how it is affecting populations at the local level. Theoretically, by taking a water sample analyze the DNA fragments from organic matter that has been shed from animals and map out the population that resides there.…
Explanation 1 (2 point) Too little of a DNA sample was loaded into the agarose gel therefore the concentration of DNA was too low to allow amplification. To fix this increase your sample size of DNA. You may have also forgotten to load the dye into the sample, sample loading dye (which gives density to hold the DNA in the well) which is this case you made a mistake in loading the gel. Explanation 2 (2 point) Perhaps your primer did not anneal to the sequence because it is the wrong primer, or you have a contamination in your original sample.…
PCR History The invaluable molecular technique known as PCR, or the polymerase chain reaction, was discovered by a biochemist named Kary Mullis. Mullis was doing research in the early 1980s at a biotechnology company in California when he initially thought of the idea regarding PCR. PCR was developed by a combination of several techniques already in existence; the synthesis of oligonucleotides and their utilization to synthesize new copies of DNA that were specific using DNA polymerases.…
Hypothesis: If I were to extract DNA without proper chemicals and equipment, the result DNA would become faulty or poor quality for further investigation My hypothesis predicts that if we were to use improper or non-lab chemicals, the result DNA would become faulty and would result in an inadequate samples for further studies, it would seem that if we were to extract DNA without chemicals like lysis solution, concentrated salt solution, resuspenstion buffer, ethanol or isopropyl alcohol, it would result in poor DNA results. Method one Method one to create a perfect result and to extract the optimal DNA, I would believe that we would need centrifuge, sample tube, micropipettors, lysis solution, concentrated salt solution, resuspenstion buffer,…
When compared to the hair of all the suspects and victim it appeared that the hair belonged to Anna Garcia. Thsw was concluded because the 3 layers cuticle, cortex, and medulla were the same shape and color from that of the crime scene. researchers know if 2 strands of hair have the same pattern or color they come from 1 source, so this would mean the hair came from Anna Garcia. Unknown Substance: The unknown substance in the crime scene was in the form of pills above Anna Garcia’s head. When taken to the lab and compared to various substances and how they reacted with indicators it was found that the unknown substance had the same reactions as Acetoxybenzoic acid or aspirin.…
On the southwest corner of 6th and Mt. Rushmore, there is a sign that says, "Welcome to Little Italy. " The building next to the sign is a quadplex that shares many small businesses. This is a "mom and pop shop" area of town. Just to the north of all these businesses is an area where most of the drug addict and alcoholic population spend their time. This was a crime infested area with king pins in all sorts of drug categories.…
DNA samples were extracted from sixteen unidentified shark tissue specimens and underwent PCR amplification of CO1 mitochondrial gene. DNA samples were assessed for presence and quality using agarose gel electrophoresis. Species identification was performed by importing finalized CO1 sequences into two DNA databases to ensure accurate identification. Phylogenic analysis was then conducted to determine relationships between identified species.…
This intellectual stimulation, coupled with a desire to discover more about life’s mysteries, compels me to pursue a career in scientifc research. This summer, I worked in a cardiology laboratory at UCLA, looking at proteins associated with HDL to understand how atherosclerosis can be averted. After some experiments provided questionable results, I was given the task of confrming that the viruses we were working with had been packaged and identifed correctly. I spent weeks running DNA gels, looking for specifc genes in each virus, but my results were inconsistent.…