After testing the D. magna with alcohol, the results showed a negative effect on the D. magna. After recording the normal heart rate in beats per minute (bpm) before the experiment, alcohol was applied to four different D. magna for each percentage of alcohol. The three concentrations of alcohol used were 5%, 10%, and 20%. Once the differences in heart rate were recorded for each of the D. magna, the averages of the four differences in heart rate for each concentration of alcohol were calculated. The averages calculated were -3 bpm for 5%, -13 bpm for 10%, and -20 bpm for 20% (Table 1.) These averages represent a decline in the heart rate of D. magna as the concentration of alcohol increased. When the averages are graphed, they form a trend
