Summary
The overall goal of this experiment was to determine if there is a connection between cyclin D1 amplification and breast cancer therapy resistance. This experiment was motivated by the statistic of forty percent of endocrine therapy breast cancer patients being resistant to the treatment. In some research, it has been thought that the presence of cyclin D1 causes resistance to tamoxifen therapy, while others do not think cyclin D1 is relevant to the efficacy of the treatment. This experiment will further the understanding of this protein and the relativity of it by performing tissue arrays of 147 breast cancer patients. It is important to find out what is causing this resistance so changes can be made and hopefully, a successful breast …show more content…
It was performed multiple times on each tumor at different parts of the tumors using FISH technology. This technology used a specific stain to fluoresce the cyclin D1 gene in one color (pink) and the centromeres of chromosomes as another color (green). Cyclin D1 amplification was present when there was a large amount of pink stain when compared to the amount of green stain, and was not present when the two quantities of the colors were relatively equal. As a result, there was only about 21% of the cancers that showed cyclin D1 amplification. It is thought that cyclin D1 amplification is an early event in the development of breast cancer due to the results concluding that the degree of amplification is highly …show more content…
It states that the purpose of this experiment was to quantify the cyclin D1 amplification and that it may have an impact on therapy resistance but does not seem to have a specific side that it is trying to prove or disprove and does not list further questions that are present that make this an important topic. This is a very important topic so more detail could have gone into trying to specify why their hypothesis is important to address. They spent a little too much time talking about previous research, rather than establishing their purpose and hypothesis for further evaluating the specific topic on cyclin
The overall goal of this experiment was to determine if there is a connection between cyclin D1 amplification and breast cancer therapy resistance. This experiment was motivated by the statistic of forty percent of endocrine therapy breast cancer patients being resistant to the treatment. In some research, it has been thought that the presence of cyclin D1 causes resistance to tamoxifen therapy, while others do not think cyclin D1 is relevant to the efficacy of the treatment. This experiment will further the understanding of this protein and the relativity of it by performing tissue arrays of 147 breast cancer patients. It is important to find out what is causing this resistance so changes can be made and hopefully, a successful breast …show more content…
It was performed multiple times on each tumor at different parts of the tumors using FISH technology. This technology used a specific stain to fluoresce the cyclin D1 gene in one color (pink) and the centromeres of chromosomes as another color (green). Cyclin D1 amplification was present when there was a large amount of pink stain when compared to the amount of green stain, and was not present when the two quantities of the colors were relatively equal. As a result, there was only about 21% of the cancers that showed cyclin D1 amplification. It is thought that cyclin D1 amplification is an early event in the development of breast cancer due to the results concluding that the degree of amplification is highly …show more content…
It states that the purpose of this experiment was to quantify the cyclin D1 amplification and that it may have an impact on therapy resistance but does not seem to have a specific side that it is trying to prove or disprove and does not list further questions that are present that make this an important topic. This is a very important topic so more detail could have gone into trying to specify why their hypothesis is important to address. They spent a little too much time talking about previous research, rather than establishing their purpose and hypothesis for further evaluating the specific topic on cyclin