Creating food: Before making the food, make sure to wash your hands thoroughly. After washing your hands, make sure to wipe down your station before using it. There are alcohol spray bottles throughout the lab. Spray it in your area and use the paper towels to wipe it down. Obtain a clean polystyrene vial and fill one eighth of the tube with dry food. The fly food was purchased from the Carolina Biological Supply company. Add about 9 ml of water to the dry food and gently roll the vial five times. Leave it untouched for two minutes so it can dry. If the food appears cracked or flakey, more water is needed. Make sure not to add too much water, otherwise the flies may drown. Use the Kimtech wipes to absorb any excess water on the sides of the
…show more content…
Collect four to six male flies from the marker vial. The marker flies used in the experiment are Curly/Plum; Dichaete/Stubble. Constantly check the vials until larvae or pupae appear. When either of those appear, remove the parents. As the pupae begin to hatch, start scoring a minimum amount of two hundred flies. Scoring is the process of looking at the flies under a microscope. Before scoring, you must turn on the CO2 so the flies stay knocked out. Turn the microscope on and set its power to max, so you can see the flies. Zoom in as much as possible since this is a bristle mutation. Focus so there is a clear visual. As scoring occurs, be sure to collect a minimum of ten virgin flies. In parallel to this, run a Marker DC1 Control Cross. Collect five to eight virgin marker flies with four to six male marker flies. This is to ensure that that a 1:1:1:1 segregation occurs in the progeny. Discard any unnecessary progeny.
Data Analysis of Discriminant Cross 1: Discriminant Cross 1 (DC1) helps identify the phenotypic class of the mutant allele. As progeny began to appear in the cross, we began keeping a count of how many flies per mutations were produced in the laboratory notebook. After we collected over 200 progenies, we counted how many of each phenotype was shown and conducted a chi-square test with the aid of Microsoft Excel. A chi-square test allows scientists …show more content…
The marker chromosomes used in the experiment are Cy, Pm, D, Sb. Cy and Pm are located on chromosome 2. D and Sb are located on chromosome 3. 66 out of 263 flies (25%) of the marker DC1 cross was Cy/D. 61 out of 263 flies (23%) are Cy/Sb. 65 out of 263 flies (25%) are Pm/D. 71 out of 263 flies (27%) are Pm/Sb. A simple chi-square test reveals a value of .77 which is low enough for us to be able to accept that there was an equal distribution of the marker alleles. It is safe to say that the observed distribution has a 1:1:1:1 ratio. This confirms that there were no mistakes made with the marker flies that could have created any invalid results in the future
Collect four to six male flies from the marker vial. The marker flies used in the experiment are Curly/Plum; Dichaete/Stubble. Constantly check the vials until larvae or pupae appear. When either of those appear, remove the parents. As the pupae begin to hatch, start scoring a minimum amount of two hundred flies. Scoring is the process of looking at the flies under a microscope. Before scoring, you must turn on the CO2 so the flies stay knocked out. Turn the microscope on and set its power to max, so you can see the flies. Zoom in as much as possible since this is a bristle mutation. Focus so there is a clear visual. As scoring occurs, be sure to collect a minimum of ten virgin flies. In parallel to this, run a Marker DC1 Control Cross. Collect five to eight virgin marker flies with four to six male marker flies. This is to ensure that that a 1:1:1:1 segregation occurs in the progeny. Discard any unnecessary progeny.
Data Analysis of Discriminant Cross 1: Discriminant Cross 1 (DC1) helps identify the phenotypic class of the mutant allele. As progeny began to appear in the cross, we began keeping a count of how many flies per mutations were produced in the laboratory notebook. After we collected over 200 progenies, we counted how many of each phenotype was shown and conducted a chi-square test with the aid of Microsoft Excel. A chi-square test allows scientists …show more content…
The marker chromosomes used in the experiment are Cy, Pm, D, Sb. Cy and Pm are located on chromosome 2. D and Sb are located on chromosome 3. 66 out of 263 flies (25%) of the marker DC1 cross was Cy/D. 61 out of 263 flies (23%) are Cy/Sb. 65 out of 263 flies (25%) are Pm/D. 71 out of 263 flies (27%) are Pm/Sb. A simple chi-square test reveals a value of .77 which is low enough for us to be able to accept that there was an equal distribution of the marker alleles. It is safe to say that the observed distribution has a 1:1:1:1 ratio. This confirms that there were no mistakes made with the marker flies that could have created any invalid results in the future