Spectrophotometer

Emily Astarita, Amelia Grose, Natalie Krapcho and I’s project is titled “Concentration Quantification and Comparison with the HIC Eluate and Bacterial Lysate.” The overall objectives of this experiments include using a spectrophotometer to obtain absorbance of standard solutions to create a standard curve from this data, and subsequently using the equation of the curve to find the concentration of GFP elute which was purified from the column. Using the spectrophotometer to create a standard curve and running samples through a chromatography column were techniques learned previously in this course, but the concept of an HIC column was a novel concept that we learned about in this experiment.
Prompt 2 The hypothesis we were testing concerned when the GFP would elute from the column based on its …show more content…
This experiment’s aim was to determine if the apples in North Dining Hall (NDH) are genetically modified. Their prediction stated that the organic apple would not contain GMOs, but the apples from NDH would contain GMOs. First, they extracted DNA from the food samples by weighing the samples and then grinding them into a slurry. Next, this group used PCR to amplify the GMO sequences and sample DNA. Lastly, they added dye to the samples, ran electrophoresis with a 3% gel, and imaged the resulting gel using the GelDoc machine. In this image, the plant primer band appeared in the non GMO control group, the GMO (+) DNA, the organic whole apple, and the pre-sliced apple. In addition, there was no GMO primer band in the non GMO control group or GMO (+) DNA (also, band did not electrophorese in organic apple), but there was a GMO band in the pre-sliced apple. Thus, their hypothesis was supported in that the NDH pre-sliced apples contain GMOs. Overall, this presentation was well done. In regard to their presentation, there was an appropriate amount of words on the slides, and the multitude of pictures was