Introduction
The microscope is an optical instrument that produces large images of small objects that cannot be observed by the human naked eye. There are two types of light microscopes: compound microscope and Electron microscope. Light microscopes are divided into two categories: Compound microscopes and Stereoscope microscopes. Electron microscope functions in the following way; a stream of electrons emitted from an electron source built in the microscope, the obtained image is in black and white. The stereoscope microscope functions by reflected light. The compound light microscope, which is going to be used in these experiments, functions by transmitted light that traverse the specimen.
Three small experiments were done. The purposes …show more content…
The relation between the magnification and the working is inversely proportional; when the magnification is bigger the working distance becomes smaller.
Experiment 2: field of view of the high-power objective
Materials:
Compound microscope Transparent ruler
Procedures:
Use a transparent ruler that serves as a specimen. Turn on the illuminator of the microscope, use the nosepiece to put the scanning objective (4X) magnification. Place the ruler on the stage. Move the stage clips so that the ruler is in the center. To obtain a clear image, use at first the coarse adjustment knob and then use the fine adjustment knob to make final changes in bringing the slide into sharp focus.
Results: The image obtained looked close to the figure shown below: (the distance between two short lines is 1 mm) As shown in the image above the diameter of the field is 4 mm. Each time the objective was moved to the next objective, the field of view got smaller. For the low-power objective, the diameter of the field was 2
The microscope is an optical instrument that produces large images of small objects that cannot be observed by the human naked eye. There are two types of light microscopes: compound microscope and Electron microscope. Light microscopes are divided into two categories: Compound microscopes and Stereoscope microscopes. Electron microscope functions in the following way; a stream of electrons emitted from an electron source built in the microscope, the obtained image is in black and white. The stereoscope microscope functions by reflected light. The compound light microscope, which is going to be used in these experiments, functions by transmitted light that traverse the specimen.
Three small experiments were done. The purposes …show more content…
The relation between the magnification and the working is inversely proportional; when the magnification is bigger the working distance becomes smaller.
Experiment 2: field of view of the high-power objective
Materials:
Compound microscope Transparent ruler
Procedures:
Use a transparent ruler that serves as a specimen. Turn on the illuminator of the microscope, use the nosepiece to put the scanning objective (4X) magnification. Place the ruler on the stage. Move the stage clips so that the ruler is in the center. To obtain a clear image, use at first the coarse adjustment knob and then use the fine adjustment knob to make final changes in bringing the slide into sharp focus.
Results: The image obtained looked close to the figure shown below: (the distance between two short lines is 1 mm) As shown in the image above the diameter of the field is 4 mm. Each time the objective was moved to the next objective, the field of view got smaller. For the low-power objective, the diameter of the field was 2