Lansoprazole Microparticles Lab Report

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Table 1 Composition of Lansoprazole microparticles
Formulation code Amount of drug(mg) Amount of polymer chitosan (mg) Drug polymer
Ratio Tween
80 (%w/v)
F1 100 100 1:1 1.25
F2 100 200 1:2 1.25
F3 100 300 1:3 1.25
F4 100 400 1:4 1.25
F5 100 500 1:5 1.25
F6 100 600 1:6 1.25

Characterization of Lansoprazole microparticle
The microparticle will be characterized for following parameters: Percentage yield: After complete drying, the drug loaded microparticle was collected and weighed accurately [9]. The yield of microparticle was calculated by:
Percentage yield =( Total wt.of formulation (g))/( Total wt.of drug +Total wt.of excipient) X100 Surface Morphology [10]: The morphology of the microparticle was determined with the aid of Scanning Electron
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STEARATE 5 5 5 5 5 5 5 5 5 5
MCC 5 5 5 5 5 5 5 5 5 5
TOTAL WEIGHT 30 30 30 30 30 30 30 30 30 30

Evaluation of tablets Pre-compression parameters Angle of repose: Angle of repose was determined by funnel method. Accurately weighed 10 g granules were taken in the funnel. Height of the funnel was adjusted such that the tip of the funnel just touches the apex of the heap of powder. The powder was allowed to flow through funnel freely onto the surface. The diameter of the powder cone was measured and angle of repose was calculated using the following equation [12]. tan Ɵ = h/r where, Ɵ= angle of repose, h= height of cone, r= radius of cone base. Bulk density: Take about 100 gm of lansoprazole microparticles into a dry 250 ml cylinder. The cylinder was filled carefully and level of powder was adjusted without compacting and the unsettled apparent volume (Vo) was noted. Bulk density was calculated, in g/ml [13]. By using the formula:
Bulk density = M/Vo
Where, M = weight of the sample taken, Vo = bulk volume Tapped density: Accurately weighed quantity of powder was introduced into a measuring cylinder. The cylinder was tapped 500 times and the tapped volume (Va) was measured [12]. Then the tapped density was calculated using the following
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Tablets thickness [13]: Tablets thickness was measured by using Vernier calliper. Five tablets was taken and placed between the two arms of the Vernier calliper.
2. Tablet hardness [14]: The crushing strength of tablets was measured by Pfizer hardness tester.
3. Friability [13, 14]: Roche friabilator was used for testing the friability. Twenty tablets was weighed accurately and placed in the tumbling apparatus that revolves at 25 rpm. After 4 min, the tablets were weighed and percentage loss in tablet weight was determined.
4. Weight Variation [15, 16]: Twenty tablets were weighed individually and the average weight was determined. Then percentage deviation from the average weight was calculated.
5. Estimation of drug content: 10 tablets were finely powdered and quantity of the powder equivalent to 30 mg of lansoprazole was accurately weighed and transferred to 250 ml volumetric flask containing 50 ml of pH 6.8 phosphate buffer and allowed to stand for 8 hour with intermittent shaking to ensure complete solubility of the drug. The solution was then made up to 100ml volume with pH 6.8 phosphate buffer and mixed thoroughly. The solution was filtered, diluted and drug content was estimated by UV spectrophotometer at 260nm.The drug concentration was calculated from the calibration curve

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