Complications Of HL 60 Cells

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HL-60 cells were derived from the blood cell of a patient with acute promyelocytic leukemia, which proliferates continuously. They are immortal cells, until they induce differentiation using chemical reagents such as retinoic acid. After differentiation, they are no longer immortal. Retinoic acid is derived from Vitamin A, and can induce granulocytes. We are going to test whether exposure of longer time to retinoic acid will induce greater percent of granulocytic differentiation. To test viability, we are going to use trypan blue and then we are going to perform May-Grunwald Staining Protocol to view cells morphologically. We found out that percentage of differentiation is directly related to the exposure of time. Retionic acid is involved …show more content…
HL-60 cells are multipotent, which means that they are able to form multiple cell types but are restricted to a specific lineage (Cirtain et al., 2016). HL- 60 cell line has been used as an in vitro system for studying cellular and molecular mechanisms involved in the proliferation and differentiation of normal and leukemic cells of the hematopoietic, or white blood cell lineage (Xie et al., 1998). HL-60 cells are invaluable as they are easy to maintain, differentiate and can be used to investigate cell development, or gene expression. HL-60 cells can be used to induce differentiation via signal transduction into monocytes, granulocytes, and lymphocytes using various chemical agents (Cirtain et al., 2016). HL-60 cells are also used to test differentiation by viewing the cell morphology and apoptosis. After differentiation, HL-60 cells are no longer immortal and undergo an apoptosis. Apoptosis is a naturally occurring programmed cell death (Cirtain et al., …show more content…
We concluded that HL-60 cells exposed to retinoic acid for 24 hour have more monocyte differentiation than 3 hour. We also found there was not a single differentiated cell for treatment with no retinoic acid as predicted. These data supports our hypothesis, while refutes the null hypothesis. Continuous exposure to retinoic acid was necessary for differentiation, with the percentage of mature cells in the culture directly related to the time of retinoic acid exposure (Breitman et al., 1980). We also found that retinoic acid also induces not only granulocytes, but also monocytes. We observed an average of 32.18 % monocytes in 24 hour treatment, and average of 68.18% monocyte in 3 hour treatment. We also saw that the percent viability decreased when cells were being treated with reagent, retinoic acid, as they were no longer immortal. It caused cell death. The reduction of percent viability also suggests that the differentiation has occurred. As there was no cell differentiation in the absence of retinoic acid, the percent viability was almost the

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