ABSTRACT: In this experiment, we determined the relative polarity of substances using the principles of chromatography. Three substances were tested using a thin layer chromatography. The column chromatography tested a mixture of two substance which were separated as a result of the chromatography. The results for both the thin layer and column chromatography supported the hypothesis. Therefore, chromatography is an effective tool that can be used to determine relative polarities.
INTRODUCTION: Chromatography is a laboratory procedure that operates on the principles of polarity using a stationary and mobile phase. Although there are several types of chromatography, this experiment focuses on thin layer and column chromatography. Thin layer …show more content…
On two slides, the one half of the slide should be labeled A and spot three small drops of acetanilide should be placed on the application point above the letter A. Do the same on the other side of the slide for Ts, trans-stilbene. On the other two slides label the one side C for 1,2-dimethoxybenzene and the other side D for a 1,2-dimethoxybenzene previously mentioned chemicals. Place one of each kind of slide in a beaker of 9:1 hexane-ethyl acetate place the other two slides in a beaker of 1:1 hexane-ethyl acetate. Allow the solvents in the jar to move up the TLC slides until the solvents are about 1 cm away from the top of the TLC slide and mark the solvent front or the point at which the solvent rises. Take the slides out the jars and let them dry before displaying the spots using a UV light. Lightly label the spots that you can see under the UV light and use this to calculate the retention factor …show more content…
Next, pour a slushy mixture of alumina in ethanol down the pipette ensuring the alumina is covered in ethanol and that there are no air bubbles in the mixture. When there is a thin layer of ethanol above the alumina, about 2-3 mm, drop three drop of a 5% methylene blue and fluorescein and 95% ethanol into the column, then pour more ethanol into the pipette while collecting the drippings in a beaker. When all the methanol blue is out of the pipette, pour water into the pipette to collect the fluorescein in a
INTRODUCTION: Chromatography is a laboratory procedure that operates on the principles of polarity using a stationary and mobile phase. Although there are several types of chromatography, this experiment focuses on thin layer and column chromatography. Thin layer …show more content…
On two slides, the one half of the slide should be labeled A and spot three small drops of acetanilide should be placed on the application point above the letter A. Do the same on the other side of the slide for Ts, trans-stilbene. On the other two slides label the one side C for 1,2-dimethoxybenzene and the other side D for a 1,2-dimethoxybenzene previously mentioned chemicals. Place one of each kind of slide in a beaker of 9:1 hexane-ethyl acetate place the other two slides in a beaker of 1:1 hexane-ethyl acetate. Allow the solvents in the jar to move up the TLC slides until the solvents are about 1 cm away from the top of the TLC slide and mark the solvent front or the point at which the solvent rises. Take the slides out the jars and let them dry before displaying the spots using a UV light. Lightly label the spots that you can see under the UV light and use this to calculate the retention factor …show more content…
Next, pour a slushy mixture of alumina in ethanol down the pipette ensuring the alumina is covered in ethanol and that there are no air bubbles in the mixture. When there is a thin layer of ethanol above the alumina, about 2-3 mm, drop three drop of a 5% methylene blue and fluorescein and 95% ethanol into the column, then pour more ethanol into the pipette while collecting the drippings in a beaker. When all the methanol blue is out of the pipette, pour water into the pipette to collect the fluorescein in a