Abstract
In this study, a CO2-respensive GM-AD-HA hydrogel derived from electrostatics crosslinks and supramolecular bond will be produced and studied about its mechanical properties and physiological performance. It is expected to have application in restoring cartilage and controllable delivery of Epirubicin
Background of research
Hydrogels have crosslinked microstructure and can swell up in water without resolving. Their soft and rubbery nature minimizes inflammatory reactions of the surrounding cells, and have a good prospect in biomedical applications such as clinic repair material or studying interaction between implanted materials by imitating extracellular matrix.1 Some hydrogels are potential to be injected as they experience viscous …show more content…
The specific process refers to this document.17 The GMHA polymers will prepared by treating a 1% w/v solution of fermentation-derived HA in distilled water with excessive glycidyl methacrylate in the presence of excess trimethylamine and tetra butyl ammonium bromide overnight at room temperature, followed by a 1h incubation at 60°C. After the reaction, the solution was precipitated in and dissolved in distilled water twice to remove excess reactants. The GMHA solution was lyophilized and stored desiccated at 4°C.
Secondly, according to the article7, GMHA, 1-adamantaneacetic acid, 4-dimethylaminopyridine will dissolve in anhydrous DMSO at room temperature, and di-tert-butyl dicarbonate will be slowly added under nitrogen protection at room temperature. The mixture will be heated to 45℃ and kept for 24 h. After cooling down to room temperature, the reaction mixture was directly dialyzed against DMSO, Na Cl(aq), and then DI water to remove all unreacted small organic compounds. The solution will be then frozen and
In this study, a CO2-respensive GM-AD-HA hydrogel derived from electrostatics crosslinks and supramolecular bond will be produced and studied about its mechanical properties and physiological performance. It is expected to have application in restoring cartilage and controllable delivery of Epirubicin
Background of research
Hydrogels have crosslinked microstructure and can swell up in water without resolving. Their soft and rubbery nature minimizes inflammatory reactions of the surrounding cells, and have a good prospect in biomedical applications such as clinic repair material or studying interaction between implanted materials by imitating extracellular matrix.1 Some hydrogels are potential to be injected as they experience viscous …show more content…
The specific process refers to this document.17 The GMHA polymers will prepared by treating a 1% w/v solution of fermentation-derived HA in distilled water with excessive glycidyl methacrylate in the presence of excess trimethylamine and tetra butyl ammonium bromide overnight at room temperature, followed by a 1h incubation at 60°C. After the reaction, the solution was precipitated in and dissolved in distilled water twice to remove excess reactants. The GMHA solution was lyophilized and stored desiccated at 4°C.
Secondly, according to the article7, GMHA, 1-adamantaneacetic acid, 4-dimethylaminopyridine will dissolve in anhydrous DMSO at room temperature, and di-tert-butyl dicarbonate will be slowly added under nitrogen protection at room temperature. The mixture will be heated to 45℃ and kept for 24 h. After cooling down to room temperature, the reaction mixture was directly dialyzed against DMSO, Na Cl(aq), and then DI water to remove all unreacted small organic compounds. The solution will be then frozen and