Cloning Of Dolly Sheep

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Register to read the introduction… This procedure starts with the removal of the chromosomes from an egg to create an enucleated egg. The chromosomes are replaced with a nucleus taken from a somatic (body) cell of the individual or embryo to be cloned. This cell could be obtained directly from the individual, from cells grown in culture, or from frozen tissue. The egg is then stimulated, and in some cases it starts to divide. If that happens, a series of sequential cell divisions leads to the formation of a blastocyst, or preimplantation embryo. The blastocyst is then transferred to the uterus of an animal. The successful implantation of the blastocyst in a uterus can result in its further development, culminating sometimes in the birth of an animal. This animal will be a clone of the individual that was the donor of the nucleus. Its nuclear DNA has been inherited from only one genetic …show more content…
DOLLY’S FACT SHEET

Dolly (Sheep) | | Dolly’s remains exhibited at the National Museum of Scotland | Species | Domestic Sheep | Sex | Female | Born | 5 July 1996 | Died | 14 February 2003 (aged 6)
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Kuehn Goodbye, Dolly; first cloned sheep dies at six years old American Veterinary Medical Association, 15 April 2003

COLMAN A. Somatic cell nuclear transfer in mammals: Progress and applications. Cloning 1999, 1(4):185-200

CHAN AW, DOMINKO T, LUETJENS CM, NEUBER E, MARTINOVICH C, HEWITSON L, SIMERLY CR, SCHATTEN GP. Clonal propagation of primate offspring by embryo splitting. Science 2000 Jan 14, 287(5451):317-319

Campbell KH, McWhir J , Ritchie WA, Wilmut I (1996). Sheep cloned by nuclear transfer from a cultured cell line. Nature 380 (6569):

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