John Salkeld
BIOL:1411:0A06
10/5/2016
Team Members: Thomas Eickhoff, Yvette Manzanares, Isara Paul Emile
I. Question & Hypothesis
Can chloroplasts be identified with light? And if so, which test tube would the light be most helpful to? We were interested in seeing where the chloroplasts ended up by adding an isolation buffer and DCIP or water.
Our hypothesis was that the chloroplasts were going to be identified with the use of light. We also believed that the chloroplasts were going to be in the P2 test tube. We knew that the chloroplasts weren’t going to be in P1. We knew this, because the chloroplast isn’t a big organelle in the cell, and the first centrifuge was designed to drag down the heavy organelles, which didn’t apply. The second centrifuge was at a faster speed, which is where we believed the chloroplasts would end up. We also knew we could figure this out with the use of light to activate the chloroplasts.
Does the color of light move the experiment more than others? Is there a better color for plants to grow by? We were interested in seeing if the color change of light would better in moving the reaction forward.
Our hypothesis was that the different colors moved the experiment in the same direction but different lengths. We thought that the color green would move the experiment the least, while the blue …show more content…
This was expected, mainly because the white light contains all of the lights together. Dividing up the white light, the blue section was what moved the experiment the furthest, by a difference of .23 nm, only .03 difference off of the white light. The following light was red light, which only decreased by 0.09 nm. The lowest change was green light, creating a change of only 0.04 nm. Looking at figure 4b, you can see how the differences compare to each other. While 4a shows the change in difference as well, it also shows where each change happened at what given
BIOL:1411:0A06
10/5/2016
Team Members: Thomas Eickhoff, Yvette Manzanares, Isara Paul Emile
I. Question & Hypothesis
Can chloroplasts be identified with light? And if so, which test tube would the light be most helpful to? We were interested in seeing where the chloroplasts ended up by adding an isolation buffer and DCIP or water.
Our hypothesis was that the chloroplasts were going to be identified with the use of light. We also believed that the chloroplasts were going to be in the P2 test tube. We knew that the chloroplasts weren’t going to be in P1. We knew this, because the chloroplast isn’t a big organelle in the cell, and the first centrifuge was designed to drag down the heavy organelles, which didn’t apply. The second centrifuge was at a faster speed, which is where we believed the chloroplasts would end up. We also knew we could figure this out with the use of light to activate the chloroplasts.
Does the color of light move the experiment more than others? Is there a better color for plants to grow by? We were interested in seeing if the color change of light would better in moving the reaction forward.
Our hypothesis was that the different colors moved the experiment in the same direction but different lengths. We thought that the color green would move the experiment the least, while the blue …show more content…
This was expected, mainly because the white light contains all of the lights together. Dividing up the white light, the blue section was what moved the experiment the furthest, by a difference of .23 nm, only .03 difference off of the white light. The following light was red light, which only decreased by 0.09 nm. The lowest change was green light, creating a change of only 0.04 nm. Looking at figure 4b, you can see how the differences compare to each other. While 4a shows the change in difference as well, it also shows where each change happened at what given