Citrus is an important horticultural plant in theworld, member of family Rutaceae refers to all edible and rootstock species and a few closely related genera. India in the world is fifth largest nations of citrus fruit producer 3. Citrus is native to India and is used as a rootstock to commercially important citrus cultivars of Citrus reticulata and Citrus sinensis Singh et al.10. Citrus fruits are grown throughout the world and are known for their fine flavor and quality.Citrus is the number one fruit of the worldwide due to its high nutritional value, considerable production of fruit, fruit products and the citrus industry is considered to be major fruit industry. Citrus varieties are propagated by both sexual and asexual methods. Generally,
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These seeds were used as explants from donor plants during the present study. These seeds were carefullywashed in running tap water for 7 minutes and followed by distilled water for 5 minutes. For surface sterilization, chemical such as 70% ethanol and Hgcl2 (0.3 %) were used. seeds were surface sterilized for 1 minute in 70% ethanol after the one minute these seeds are also sterilizing with 0.3% Hgcl2(mercuric chloride) for 3 minute followed by three subsequent rinses with sterilized double distilled water in a laminar airflow. All these seeds were dissected and removing the seed coat carefully and aseptically inoculated in test tube as well as culture vessels containing 25 ml of MS (Murashige & Skoog) medium contain 3% sucrose and solidified agent Clerigel with 0.3%, for germination of seeds with various concentrations of growth …show more content…
reticulata. These explant examined MS medium supplemented with different concentration and combination of growth regulators like, BAP, KIN, GA3, IAA and various concentration of amino acid. MS medium containing with 3% sucrose and gelled 0.3% Clerigel solidified agent and after the adding of growth regulators the pH was adjusted 5.8. Then media was steam sterilized in an autoclave under 15 psi pressures and 121° C temperature. After theautoclavethese media was transfer to laminar air flow for solidified and inoculation of explant. For germination of seeds culture vessels were incubated in darkness at a constant 25 °C temperature for 15 Days and, in a growth chamber with 16 hours of photoperiod and 60 % relative humidity for 3 weeks 8. Shoot, leaf and nodal segments explants were excised from 5-week-old in vitro grown seedlings cut into 0.5 cm pieces for studies of multiple shoots formation as well as callus initiation by addingvarious concentration of growth regulators (BAP, IAA). Inoculated culture tubes and culture vessels were transfers to culture room under a 16 hours photoperiod supplied by cool white fluorescent tubes light and temperature 25 ± 0C.Data was measured after 30 days of five replicate mean ±
These seeds were used as explants from donor plants during the present study. These seeds were carefullywashed in running tap water for 7 minutes and followed by distilled water for 5 minutes. For surface sterilization, chemical such as 70% ethanol and Hgcl2 (0.3 %) were used. seeds were surface sterilized for 1 minute in 70% ethanol after the one minute these seeds are also sterilizing with 0.3% Hgcl2(mercuric chloride) for 3 minute followed by three subsequent rinses with sterilized double distilled water in a laminar airflow. All these seeds were dissected and removing the seed coat carefully and aseptically inoculated in test tube as well as culture vessels containing 25 ml of MS (Murashige & Skoog) medium contain 3% sucrose and solidified agent Clerigel with 0.3%, for germination of seeds with various concentrations of growth …show more content…
reticulata. These explant examined MS medium supplemented with different concentration and combination of growth regulators like, BAP, KIN, GA3, IAA and various concentration of amino acid. MS medium containing with 3% sucrose and gelled 0.3% Clerigel solidified agent and after the adding of growth regulators the pH was adjusted 5.8. Then media was steam sterilized in an autoclave under 15 psi pressures and 121° C temperature. After theautoclavethese media was transfer to laminar air flow for solidified and inoculation of explant. For germination of seeds culture vessels were incubated in darkness at a constant 25 °C temperature for 15 Days and, in a growth chamber with 16 hours of photoperiod and 60 % relative humidity for 3 weeks 8. Shoot, leaf and nodal segments explants were excised from 5-week-old in vitro grown seedlings cut into 0.5 cm pieces for studies of multiple shoots formation as well as callus initiation by addingvarious concentration of growth regulators (BAP, IAA). Inoculated culture tubes and culture vessels were transfers to culture room under a 16 hours photoperiod supplied by cool white fluorescent tubes light and temperature 25 ± 0C.Data was measured after 30 days of five replicate mean ±