Experiment 3 titled “Physiologically Important IONS” and the purpose of this lab is to become familiar with metal and nonmetal ions and mostly important in biological and physiological processes. Also, to become familiar with tests to determine the presence or absence of ions as well as to estimate approximate amount. To begin, when a solution is tested, make sure you observe everything and note everything. This is lab, we will test some positive ion and determines if it’s an anion or a cation. To go with that, we will check its properties size, shape, charge, color, and chemical behaviors. During the experiment, correct terminology must be used in the recording of observations because we will be testing unknown solutions containing ion. Unknown
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First, have a wire loop for the flame test and adjust the burner so it is blue and no-luminous. Then, clean the wear loop by dipping it in concentrated hydrochloric acid and heat the flame until it glows. Pour about 10 milliliters of 10% sodium chloride solutions into a small beaker and for unknown use 2mL of unknown solution instead of sodium chloride. Dip the clean wire loop into sodium chloride solution and place the loop in the flame and record the observation. Third, potassium flame will be tested. First, clean the wire lop with concentrated HCI for sodium flame test. Pour two milliliters of 10% potassium chloride solution into a small beaker and for unknow use 2 ml of unknown solution. Dip the wire into potassium chloride solution and test the flame. Observe the color of the flame and record it on the data sheet. Fourth, calcium, pour one ml of 0.1 M Ca(NO3)2 into a test tube and add few drop of 0.1 M Na2C2O2 solution. Warm the mixture, cool to room temperature, and record the observation. Fifth, zinc, place one ml of 0.1 M zinc nitrate solution into a test tube and add drops of 6M ammonium chloride solution. Add one drop of ammonium hydroxide and thioacetamide and heat it on the water …show more content…
Lastly, ammonium ion, place two ml ammonium nitrate into a test tube and add few drops of sodium hydroxide. After that, place a paper at the mouth of the test tube and gently heat it. Make sure not to let the solution boil and do not hold the test tube under your nose. To begin the experiment with the anions, chloride, first place one ml of sodium chloride into a test tube and add few drops of silver nitrate. After that, chloride ion combines with silver ion to form a white precipitate. Record the observation. Second, phosphate, place one ml sodium phosphate into a test tube, add one ml of nitric acid, and add one ml of ammonium molybdate. Mix it thoroughly and place it into boiling water. Remove the test tube and record the observation. Third, sulfate, place one milliliter sodium sulfate into a test tube, add one ml hydrochloric acid, and add 3-4 drops of barium chloride. Warm it in a water and cool it for serval minutes. Record your observation. Lastly, carbonate, place a small portion of sodium carbonate into a test tube and place it in a rack. Add 3-4 drops of hydrochloric acid to cover the solid. Stand and observe and record it on the data
First, have a wire loop for the flame test and adjust the burner so it is blue and no-luminous. Then, clean the wear loop by dipping it in concentrated hydrochloric acid and heat the flame until it glows. Pour about 10 milliliters of 10% sodium chloride solutions into a small beaker and for unknown use 2mL of unknown solution instead of sodium chloride. Dip the clean wire loop into sodium chloride solution and place the loop in the flame and record the observation. Third, potassium flame will be tested. First, clean the wire lop with concentrated HCI for sodium flame test. Pour two milliliters of 10% potassium chloride solution into a small beaker and for unknow use 2 ml of unknown solution. Dip the wire into potassium chloride solution and test the flame. Observe the color of the flame and record it on the data sheet. Fourth, calcium, pour one ml of 0.1 M Ca(NO3)2 into a test tube and add few drop of 0.1 M Na2C2O2 solution. Warm the mixture, cool to room temperature, and record the observation. Fifth, zinc, place one ml of 0.1 M zinc nitrate solution into a test tube and add drops of 6M ammonium chloride solution. Add one drop of ammonium hydroxide and thioacetamide and heat it on the water …show more content…
Lastly, ammonium ion, place two ml ammonium nitrate into a test tube and add few drops of sodium hydroxide. After that, place a paper at the mouth of the test tube and gently heat it. Make sure not to let the solution boil and do not hold the test tube under your nose. To begin the experiment with the anions, chloride, first place one ml of sodium chloride into a test tube and add few drops of silver nitrate. After that, chloride ion combines with silver ion to form a white precipitate. Record the observation. Second, phosphate, place one ml sodium phosphate into a test tube, add one ml of nitric acid, and add one ml of ammonium molybdate. Mix it thoroughly and place it into boiling water. Remove the test tube and record the observation. Third, sulfate, place one milliliter sodium sulfate into a test tube, add one ml hydrochloric acid, and add 3-4 drops of barium chloride. Warm it in a water and cool it for serval minutes. Record your observation. Lastly, carbonate, place a small portion of sodium carbonate into a test tube and place it in a rack. Add 3-4 drops of hydrochloric acid to cover the solid. Stand and observe and record it on the data