Abstract:
Chemiluminescence is an essential topic in the study of chemistry. The reaction involves mixing two chemicals were to form an excited (high-energy) intermediate that releases energy as light. This experiment first synthesized luminol by reducing 5-nitro-2,3-dihyodropthlazine-1,4-dione, using sodium hydrosulfite (Na2S2O4) and 3M sodium hydroxide (NaOH). The amount of luminol collected was 0.2693 g with the percent yield of 161%. The product of the first reaction was then used to recreate the chemiluminescence experiment by mixing luminol, oxidizing agent (hydrogen peroxide), and base (any hydroxide base). This reaction emitted a blue light along with yielding phthalate salt. The experiment showed the importance of luminol in chemiluminescence and yielded much needed knowledge about reactions and how they …show more content…
NaOH (2 mL, 3 M) was then added to the conical vial. After this, Na2S2O4 (251 mg, 1.44 mmol, 1 equivalent) was added to the solution. The solid leftover from the solution required washing with water. To further the reaction, a reflux apparatus was assembled by attaching a condenser (with water in/water out lines attached) to the conical vial and letting water flow through the system. The apparatus was then heated up to over 250 degrees Celsius (solution’s boiling point). After 5 minutes, the solution was let cool and acetic acid (1 mL, 1.05 g, 0.0167 mol) was added to the system. The solution needed to be cool down for another 10 minutes using ice. Precipitate (luminol) formed and then was filtered using a vacuum apparatus. To assemble the apparatus, a funnel fitted with filter paper was inserted into a filter flask (attached to the vacuum trap). As mixture was poured into the funnel, the vacuum drew out liquid and the solid could dry. The resulting weight of the product is then
Chemiluminescence is an essential topic in the study of chemistry. The reaction involves mixing two chemicals were to form an excited (high-energy) intermediate that releases energy as light. This experiment first synthesized luminol by reducing 5-nitro-2,3-dihyodropthlazine-1,4-dione, using sodium hydrosulfite (Na2S2O4) and 3M sodium hydroxide (NaOH). The amount of luminol collected was 0.2693 g with the percent yield of 161%. The product of the first reaction was then used to recreate the chemiluminescence experiment by mixing luminol, oxidizing agent (hydrogen peroxide), and base (any hydroxide base). This reaction emitted a blue light along with yielding phthalate salt. The experiment showed the importance of luminol in chemiluminescence and yielded much needed knowledge about reactions and how they …show more content…
NaOH (2 mL, 3 M) was then added to the conical vial. After this, Na2S2O4 (251 mg, 1.44 mmol, 1 equivalent) was added to the solution. The solid leftover from the solution required washing with water. To further the reaction, a reflux apparatus was assembled by attaching a condenser (with water in/water out lines attached) to the conical vial and letting water flow through the system. The apparatus was then heated up to over 250 degrees Celsius (solution’s boiling point). After 5 minutes, the solution was let cool and acetic acid (1 mL, 1.05 g, 0.0167 mol) was added to the system. The solution needed to be cool down for another 10 minutes using ice. Precipitate (luminol) formed and then was filtered using a vacuum apparatus. To assemble the apparatus, a funnel fitted with filter paper was inserted into a filter flask (attached to the vacuum trap). As mixture was poured into the funnel, the vacuum drew out liquid and the solid could dry. The resulting weight of the product is then