Chemical Aspects of Digestion Lab Report
By Abdulelah Almutairi
Animal Form and Function Lab, 03, 12:30 PM
Instructor: Melanie Gustafso-Ropski – Lab Assistant: Corinna
Monday March 9,2015
1.
Part A
• The enzyme used in this experiment is pancreatic lipase. The substrate for pancreatic lipase is lipids or triglycerides. Hydrolysis of triglycerides occurs in the duodenum and the PH rages are from 7.1 to 8.2.
• Phenol red was used as a PH indicator of the samples. if the sample is an acid then a yellow color is appeared, if the sample is alkaline then a red color will appeared. if the sample is neutral, it will be orange or if the sample is alkaline and has a PH of 10 or less, then it will have a pink color.
Table 1 - Result of Lipid Digestion with …show more content…
2. The six major constituents of bile from highest to lowest are bile salt, phospholipids, cholesterol, bilirubin, inorganic salts, and metals. (Farina-2009) Bile is produced in the liver and stored in the gallbladder. One of the functions of bile salts is to break the fats into smaller masses for easier digestion. (Taylor-2013)
3. Heating an enzyme makes the hydrogen bonds to break from its three-dimensional shape. Then the enzyme can’t function because it needs to be in a specific shape in order to work. By placing the enzyme in ice bath as we did with tube 17, the activity of the enzyme drops and the molecular motion decreases. If the temperature rise between 40 C and 50 C, the activity of the enzyme will increase.
4. The four proteases we used are, chymotrypsinogen, trypsinogen, procarboxypeptidase, and pepsinogen. The name of zymogens for each protease in order is chymotrypsin, trypsin, carboxypeptidase, and pepsin. Chymotrypsin and trypsin are activated by enteropeptidase.
5. BOLUS is a round mass of food ready to swallow.
By Abdulelah Almutairi
Animal Form and Function Lab, 03, 12:30 PM
Instructor: Melanie Gustafso-Ropski – Lab Assistant: Corinna
Monday March 9,2015
1.
Part A
• The enzyme used in this experiment is pancreatic lipase. The substrate for pancreatic lipase is lipids or triglycerides. Hydrolysis of triglycerides occurs in the duodenum and the PH rages are from 7.1 to 8.2.
• Phenol red was used as a PH indicator of the samples. if the sample is an acid then a yellow color is appeared, if the sample is alkaline then a red color will appeared. if the sample is neutral, it will be orange or if the sample is alkaline and has a PH of 10 or less, then it will have a pink color.
Table 1 - Result of Lipid Digestion with …show more content…
2. The six major constituents of bile from highest to lowest are bile salt, phospholipids, cholesterol, bilirubin, inorganic salts, and metals. (Farina-2009) Bile is produced in the liver and stored in the gallbladder. One of the functions of bile salts is to break the fats into smaller masses for easier digestion. (Taylor-2013)
3. Heating an enzyme makes the hydrogen bonds to break from its three-dimensional shape. Then the enzyme can’t function because it needs to be in a specific shape in order to work. By placing the enzyme in ice bath as we did with tube 17, the activity of the enzyme drops and the molecular motion decreases. If the temperature rise between 40 C and 50 C, the activity of the enzyme will increase.
4. The four proteases we used are, chymotrypsinogen, trypsinogen, procarboxypeptidase, and pepsinogen. The name of zymogens for each protease in order is chymotrypsin, trypsin, carboxypeptidase, and pepsin. Chymotrypsin and trypsin are activated by enteropeptidase.
5. BOLUS is a round mass of food ready to swallow.