Essay on Change The Amount Of Enzyme

850 Words Mar 30th, 2016 4 Pages
Part B: Change the amount of enzyme

Table 4: The absorbances of 5 cuvettes that measured at 475nm during a given time interval using spectrophotometer.

Time (s) Absorbance @ 475nm Cuvette 1 Cuvette 2 Cuvette 3 Cuvette 4 Cuvette 5
0 0.003 0.003 0.004 0.002 0.009
30 0.003 0.004 0.008 0.013 0.021
60 0.005 0.005 0.013 0.016 0.031
90 0.005 0.008 0.019 0.020 0.040
120 0.007 0.011 0.022 0.033 0.051

Figure 4: The graph of time vs. absorbance of 5 cuvettes that contained different amount of enzyme.

Part C: Change the amount of substrate

Table 5: The absorbances of 5 cuvettes that measured at 475nm during a given time interval using spectrophotometer

Time (s) Absorbance @ 475nm Cuvette 1 Cuvette 2 Cuvette 3 Cuvette 4 Cuvette 5
0 0.006 0.006 0.008 0.008 0.009
30 0.012 0.018 0.014 0.016 0.015
60 0.018 0.025 0.024 0.028 0.027
90 0.023 0.033 0.034 0.036 0.041
120 0.030 0.040 0.041 0.047 0.053

Figure 5: The graph of time vs. absorbance of 5 cuvettes that had different amount of substrate.

Part D: Addition of inhibitor

Table 6: The absorbances of 5 cuvettes that measured at 475nm during a given time interval using spectrophotometer

Time (s) Absorbance @ 475nm Cuvette 1 Cuvette 2 Cuvette 3 Cuvette 4 Cuvette 5
0 0.003 0.007 0.010 0.011 0.010
30 0.005 0.011 0.015 0.015 0.015
60 0.008 0.016 0.021 0.022 0.021
90 0.012 0.022 0.027 0.031 0.032
120 0.016 0.026 0.033 0.039 0.042

Figure 6: The graph of time vs. absorbance of 5 cuvettes that had the…

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