# Essay on Change The Amount Of Enzyme

850 Words 4 Pages
Part B: Change the amount of enzyme

Table 4: The absorbances of 5 cuvettes that measured at 475nm during a given time interval using spectrophotometer.

Time (s) Absorbance @ 475nm Cuvette 1 Cuvette 2 Cuvette 3 Cuvette 4 Cuvette 5
0 0.003 0.003 0.004 0.002 0.009
30 0.003 0.004 0.008 0.013 0.021
60 0.005 0.005 0.013 0.016 0.031
90 0.005 0.008 0.019 0.020 0.040
120 0.007 0.011 0.022 0.033 0.051

Figure 4: The graph of time vs. absorbance of 5 cuvettes that contained different amount of enzyme.

Part C: Change the amount of substrate

Table 5: The absorbances of 5 cuvettes that measured at 475nm during a given time interval using spectrophotometer

Time (s) Absorbance @ 475nm Cuvette 1 Cuvette 2 Cuvette 3 Cuvette 4 Cuvette 5
0 0.006 0.006 0.008 0.008 0.009
30 0.012 0.018 0.014 0.016 0.015
60 0.018 0.025 0.024 0.028 0.027
90 0.023 0.033 0.034 0.036 0.041
120 0.030 0.040 0.041 0.047 0.053

Figure 5: The graph of time vs. absorbance of 5 cuvettes that had different amount of substrate.

Table 6: The absorbances of 5 cuvettes that measured at 475nm during a given time interval using spectrophotometer

Time (s) Absorbance @ 475nm Cuvette 1 Cuvette 2 Cuvette 3 Cuvette 4 Cuvette 5
0 0.003 0.007 0.010 0.011 0.010
30 0.005 0.011 0.015 0.015 0.015
60 0.008 0.016 0.021 0.022 0.021
90 0.012 0.022 0.027 0.031 0.032
120 0.016 0.026 0.033 0.039 0.042

Figure 6: The graph of time vs. absorbance of 5 cuvettes that had the…

## Related Documents

• ###### Essay Factors That Affect The Rate Of Reactions

Enzymes are known as catalysts that cause reactions to occur more rapidly by lowering the reaction’s activation energy. A reaction’s activation energy is known as the minimum amount of energy required to get a chemical reaction started1. In order for cells to efficiently manage thousands of different chemical reactions, they make use of biological catalysts. Without these catalysts, biological reactions would not be able to proceed at a proper rate necessary to maintain life. Most, but not all,…

Words: 751 - Pages: 4
• ###### Essay Biological Reactions Of Chemical Reactions

reactions within cells. These biological molecules are called enzymes. Enzymes are the catalysts of biological systems. Although, some enzymes represent and depend on RNA (ribonucleic acid) most are made up of amino acids making them proteins. Since there are many chemical reactions that take place within very cell of the body, enzymes are ideal when keeping these cells alive because they serve as biological catalysts [3]. Enzymes are facilitators that speed up the chemical reactions by lowering…

Words: 1628 - Pages: 7
• ###### How Different Factors Affect The Reaction Rate Of Enzymes Reacting With Their Corresponding Substrates

The purpose of conducting this experiment was to explore how different factors affect the reaction rate of enzymes reacting with their corresponding substrates in order to learn more about how enzymes function in different environments. The independent variables investigated in this experiment were the concentration of different substrates, the temperature of the environment, and the effect of a catalyst on the reaction rate. The dependent variable for all of the investigations was the time it took…

Words: 1027 - Pages: 5
• ###### Essay Lab Report On Enzyme And How The Enzyme Reacts

November 3rd, 2014 A Period Abstract: This report is a demonstration of different conditions an enzyme is exposed to and how the enzyme reacts in response. The conditions the enzyme was exposed to include: ideal conditions, the presence of a competitive substrate, denaturation for a short amount of time, and complete denaturation. The enzyme being observed was an imaginary one called “toothpickase”. This “enzyme” was to break toothpicks without any obstructions, the presence of paperclips as competition…

Words: 3076 - Pages: 13
• ###### Essay Enzyme Substrate Complex

Meera Patel Enzyme lab 6/3/2013 Assignment 1 :- Effect of Temperature on Invertase Activity, Determining the Starting Velocity. Enzyme is the fastest known catalyst. They increase the velocity and rate of reactions in living cells. Diverse range of proteins works as enzyme with their specific temperature point, pH etc. Enzymes react with the molecules known as substrates. Binding site of an enzyme and a substrate is known as active site. The amount of enzyme present in a reaction is measured…

Words: 1514 - Pages: 7
• ###### Essay How Enzymes Affect The Enzyme

Introduction Enzymes are catalysts that speed up chemical reactions without being used up in the process. Enzymes are able to do this by bringing two substrates together and having them bind to the enzyme itself. This allows the enzyme to lower the activation energy required to start the chemical reaction by forcing the substrates into an unstable transition state. In order to make sure the substrates fit, enzymes are able to mold their half-moon shape to fit around the substrates. Changing…

Words: 1798 - Pages: 8
• ###### Enzyme Catalyst Lab-Write Up Essay

The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically, the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required…

Words: 2124 - Pages: 9
• ###### Essay Enzymes And Its Effect On The Body

Enzymes are substances produced by living organisms that act as catalysts to reduce the activation energy required for a chemical reaction. By lowering the activation energy, enzymes speed up the chemical reaction by providing an alternative action pathway (“Enzymes”). Enzymes are required in minute amounts because they remain unchanged after a reaction has occurred, so the same enzyme molecules can be reused over and over again. Since enzymes are proteins, they have active sites that speed up the…

Words: 756 - Pages: 4
• ###### The Effect of Enzymes on Hydrogen Peroxide Decomposition Essay

What is the function of enzymes in a living system? Enzymes speed up metabolic reactions necessary for life. Without them certain vital processes would not take place and the body would be unable to function. Difference enzymes work better under different conditions. Where in a human body might it be beneficial to have enzymes work in very acidic environments? In areas, like the stomach, that have a pH of two would benefit by having enzymes that function well in acidic environments. An example…

Words: 840 - Pages: 4
• ###### Essay Wgu Biochemistry Task 4

features that make all enzymes catalysts . A catalyst is a substance which can accelerate the rate of a chemical reaction without going through a chemical change itself. Enzymes are proteins which help accelerate the rate of reactions. Enzymes also lower activation energy without being used. (Hudon­Miller 2012) After and enzyme and substrate bind it converts the substrate to it’s specific product. The enzyme remains unchanged and it is free to move on to other products as the enzyme itself does not undergo…

Words: 963 - Pages: 4