Importance Of Cell Culture In Molecular Biology

Cell Culture: An Important Technique in Molecular Biology In this experiment, we performed cell culture using CHO cell lines. The purpose of this experiment was to grow cells in-vitro to learn about cell culture techniques and how to apply it to molecular biology such as cancer research and the production of monoclonal antibodies and vaccines. Cell/ tissue culture is the cultivation of cells, tissues or organs from an animal, plant or insect and growing them in an artificial environment (in vitro) usually glass or plastic flask in growth media that provides essential nutrients for survival and growth. Cell culture is an imperative technique for molecular medicine which involves clinical research and medical therapies. In this lab report, I will be discussing the process of cell culture, the importance of cell culture techniques and how it is applied in the world of molecular biology. A particular process of cell …show more content…
For instance, there is mammalian, insect, and human cell culture. Mammalian cell cultures represent the major source for a number of very important biopharmaceutical products, including viral vaccines, monoclonal antibodies, and hormones. These products are usually produced in small quantities due to the complexity of mammalian cell culture conditions, their vulnerability to reduced production and cell death due to deviations in the culture conditions. CHO and HEK 93 are two important cell lines used in mammalian cell culture; important characteristics of these cells are their receptiveness to transfection, their ability to grow in suspension cultures without addition of serum that contains animal proteins, and their suitability for high-density and large-scale production. CHO cells and mouse myeloma cells are most commonly used for long-term (stable) gene expression and when high yields of heterologous proteins are