# Essay on cell bio homework 3

940 Words Nov 16th, 2013 4 Pages
Cellular Biology Lab – Homework #3
Due the week of Nov. 4th
You may use the lab manual, pre-lab lectures, and credible internet resources, however you may not use your cell bio lab classmates as a resource. You will most likely see this material again on the Final and I highly encourage you to work individually and seek help from myself or your TA. Plagiarism will result in an automatic zero.

1. In the cell bio lab, we use company manufactured gels, however you can make you own polyacrylamide gels. List all of the ingredients found in an SDS-PAGE gel. Which ingredients are responsible for polymerizing the solution? How does the percentage of acrylamide effect the migration of proteins (ex: 4% gel vs. 18% gel)?
The percent
Rf= distance traveled by protein / total distance traveled

4. In lab 9, we will use differential centrifugation to separate cauliflower cell organelles into individual fractions. Describe the basic principles of differential centrifugation. Discuss why a relatively low speed centrifuge spin is enough to separate nuclei from a cell lysate while chloroplasts and lysosomes require higher speeds to separate. Relatively Low speed centrifuge spin is enough to separate nuclei from a cell lysate because the molecular weight difference of nuclei and the cell lysate is very large. Higher Centrifuge speeds are required to separate chloroplasts and lysosomes because they have a small difference in molecular weight.

5. You need to prepare 200 uL of each protein at a concentration of 1mg/mL for an upcoming experiment. Calculate the recipe to make each desired solution at the above concentration and volume. Do you have enough materials to make each dilution? Note: You diluent is water.

a. Alpha-tubulin: you have 500 uL at 5 mg/mL
a.i. (V1)(5 mg/mL)=(200uL)( 1mg/mL ) = 40 uL of stock Alpha-tubulin solution and 160 uL of water Has enough stock solution

b. TGFbeta: you have 300 uL at 2.4 mg/mL
b.i. (V1)(2.4 mg/mL)=(200uL)( 1mg/mL )= 83.33 uL of stock TGFbeta solution and 116.67 uL of water Has enough stock solution

c. Albumin: you have 150 uL at 1 mg/mL
c.i. (V1)(1 mg/mL)=(200uL)(

## Related Documents

• ###### Acc 307 Week 3 Homework Essay

WEEK 3 HOMEWORK To purchase this visit here: http://www.activitymode.com/product/acc-307-week-3-homework/ Contact us at: SUPPORT@ACTIVITYMODE.COM ACC 307 WEEK 3 HOMEWORK ACC 307 Week 3 Homework Chapters 6,7 - NEW 2015 Version Strayer Activity mode aims to provide quality study notes and tutorials to the students of ACC 307 Week 3 Homework in order to ace their studies. ACC 307 WEEK 3 HOMEWORK To purchase this visit here: http://www.activitymode.com/product/acc-307-week-3-homework/ Contact…

Words: 616 - Pages: 3
• ###### Essay on homework 3

do the search. Read the case and answer these questions. Copy and paste this information into a Word document, include your name on that document, and answer the questions. 1. What must a party establish to prevail on a motion for summary judgment? (3 points) In order to prevail on a motion for summary judgment, a movant has the burden to demonstrate that no genuine issue of material fact remains to be litigated; that it is entitled to judgment as a matter of law, and that it appears from the evidence…

Words: 1553 - Pages: 7
• ###### Essay Homework 3

The Muscular System: Neuromuscular Junction 1. What insulates each muscle cell? __________endomysium_______________ 2. Synaptic vesicles in the axon terminal of a motor neuron contain what neurotransmitter? __________acetylcholine_______________ 3. An action potential in the axon terminal of a motor neuron opens what type of ion channels? ____________Voltage-gated Calcium_____________ 4. By what means of membrane transport does the neurotransmitter leave the axon…

Words: 830 - Pages: 4
• ###### Chapter 3: Cells Essay

BIO 165: Anatomy & Physiology I | Stanly Community College | Student Name: | Emily Mansfield | Lecture Activity #3: Cells (Chapter 3) Instructions: Read chapter 3 in your textbook and review the lecture notes and study resources provided by your instructor. Type your answer in the answer block provided for each question. Answer blocks should expand as you type. If you experience difficulty typing in the provided answer blocks, you may type your answers in a new document. Save a…

Words: 787 - Pages: 4
• ###### Essay on Cell bio hw1

Cell Biology Lab - Homework # Due the week of Feb 3rd This is NOT a lab group project. Do the work individually. You may use any source other than your fellow students. Show your work, use your own words and cite all references. 1. (14 points) The HeLa S3 cancer cell line is considered to be “continuous” due to acquired mutations that enable the cells to evade senescence. This property is one of several hallmark characteristics of cancer cell lines. Describe three hallmark characteristics…

Words: 611 - Pages: 3
• ###### Fin 571 Week 3 Individual Homework Essay

FIN 571 Week 3 Individual Homework Click Link Below To Buy: http://hwaid.com/shop/fin-571-week-3-individual-homework/ Week 3 Individual Homework Submit Through Gradebook In Excel Proforma Cash Flows I- The Martinsen Company is considering the purchase of a chemical analysis machine. Although the machine being considered will not produce any increase in sales revenues, it will result in before tax the reduction of labor costs by \$35,000 per year. The machine has a purchase price of \$100…

Words: 767 - Pages: 4
• ###### Essay Homework Chapter 3

Multiple Choice 1) Module 2) Divide and Conquer 3) Header 4) Call 5) Return 6) Top-down Design 7) Flowchart 8) Local Variable 9) Scope 10) Argument 11) Parameter 12) By Value 13) By Reference 14) Global variable 15) Global True or False 1) False 2) True 3) True 4) False 5) True 6) False 7) False 8) True 9) True 10) False 11) True 12) False Short Answers 1) You can call the module several times instead of writing it out each time. 2) The header is the starting…

Words: 1568 - Pages: 7
• ###### Essay on Bio Cells

add the hashtags #OMGHEAVEN #TURNDOWNFORWHAT? #DIABEETUSDONTCARE #FOODOFTHEGODS #NOMNOMNOM #BLESSED Hypothesis- If I consume alcohol then I WILL consume more carne asada fries then when I have not consumed alcohol. Experiment/Data- Days 1 through 3 I would go to Ra Sushi and have a couple of Sake Bombers. I will take a picture of each Sake Bomber I have and post it to my social media sights with the hashtag #DOINGSCIENCE #SMARTGUYOVERHERE #WESFAWCETTWISHESHEWASHERE This would serve as a way to…

Words: 631 - Pages: 3
• ###### Ib Bio Hl Chap 1 Cell Essay example

will contribute to the cell theory? I. II. III. A. B. C. D. I only II only I and III only I, II and III [1] Living organisms are composed of cells. All cells come from pre-existing cells by mitosis. Cells are the smallest units of life. 1. C 2. A red blood cell is 8 μm in diameter. If drawn 100 times larger than its actual size, what diameter will the drawing be in mm? A. B. C. D. 0.08 mm 0.8 mm 8 mm 80 mm [1] 2. B 3. A cell has cytoplasm, a cell wall, naked DNA and ribosomes…

Words: 9877 - Pages: 40
• ###### Week 3 Homework Essay examples

Week 3 homework Exercise 7.1, problem 10a The total number of pairs we can either include or not is 4^2-4=16. Any reflexive relation is a subset of this set of 12 elements; we know there are 2^12 such subsets. Problem 10b The number of decisions we can make for any symmetric relation is 4+ (16-4)/2=4+6=10. The number of possible symmetric relations is 210. Exercise 7.2, Problem 15a a) Draw the digraph G1 (V1, E1) where V1 {a, b, c, d, e, f } and E1 {(a, b), (a, d), (b, c), (b, e), (d, b)…

Words: 944 - Pages: 4