There are factors that can affect the enzymatic activity. Enzymes are known to speed up the biological reactions. It is specific to its substrate which in this experiment is known to be catechol. The catechol oxidase is used in all parts to express the concentration in the enzyme reacting into ortho-quinone (Schultz, 2006). The catechol oxidase if from the potato that was used in this experiment. The enzyme was extracted as the full strength after centrifugation and used the homogenization as the supplement for all parts of the experiment (Shultz, 2006). Concentration of the enzyme or the substrate can increase the reaction rate so the enzyme being added helps clarify the meaning of that with the absorbance readings once the enzyme is added
