Essay about Catechol Oxidase Lab
November 4th, 2013
Catechol oxidase is an enzyme that speeds up the oxidation reaction when catechol is exposed to oxygen. When the reaction occurs, benzoquinone is produced turning the oxidized substance brown. It was hypothesized that the higher the concentration of catechol oxidase, the browner the substance will turn, and the faster it will achieve the color. In the present lab, different concentrations of catechol oxidase were mixed with pure catechol and the rate at which each solution browned was measured using a colorimeter. The …show more content…
The independent variable was the concentration of catechol oxidase, the dependent variable was the rate of turning brown, there were no controls, there were three replications and the sample sizes were 11 (for high concentration) and 10 (for medium and low concentrations).
Figure 1 shows that before the rates began to plateau, the high enzyme concentration solution was turning brown the fastest. Table 1 shows that the high enzyme concentration had the fastest rate and that on average, it deviated from the mean more than the medium and low concentrations. Figure 2 shows that the high enzyme concentration solution was turning brown the fastest. Table 2 shows that the different rates each solution turned brown are statistically significant.
Table 1: Average Rates of for High, Medium and Low Concentrations of Catechol Oxidase and the Absolute Average Deviations.
Three different concentrations of catechol oxidase were added