Essay about Catechol Oxidase Lab

915 Words Dec 14th, 2013 4 Pages
The Effect of Different Concentrations of the Enzyme Catechol Oxidase on the Rate of Benzoquinone Production When Mixed with Pure Catechol

Carson Levine
November 4th, 2013

Abstract

Catechol oxidase is an enzyme that speeds up the oxidation reaction when catechol is exposed to oxygen. When the reaction occurs, benzoquinone is produced turning the oxidized substance brown. It was hypothesized that the higher the concentration of catechol oxidase, the browner the substance will turn, and the faster it will achieve the color. In the present lab, different concentrations of catechol oxidase were mixed with pure catechol and the rate at which each solution browned was measured using a colorimeter. The
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Both liquids were then injected into the 5mL test tube. The 5mL tube was placed into the opening in the colorimeter and the lid of the colorimeter was closed. The measure of absorbance of blue light displayed on the screen of the colorimeter was recorded every 15 seconds for five minutes. This procedure was repeated for two more trials, each with 4mL of pure catechol; one used 1mL of medium concentrated catechol oxidase and one used 1mL of low concentrated catechol oxidase.

The independent variable was the concentration of catechol oxidase, the dependent variable was the rate of turning brown, there were no controls, there were three replications and the sample sizes were 11 (for high concentration) and 10 (for medium and low concentrations).

Results Section

Figure 1 shows that before the rates began to plateau, the high enzyme concentration solution was turning brown the fastest. Table 1 shows that the high enzyme concentration had the fastest rate and that on average, it deviated from the mean more than the medium and low concentrations. Figure 2 shows that the high enzyme concentration solution was turning brown the fastest. Table 2 shows that the different rates each solution turned brown are statistically significant.

Table 1: Average Rates of for High, Medium and Low Concentrations of Catechol Oxidase and the Absolute Average Deviations.
Three different concentrations of catechol oxidase were added

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