Enzymes are proteins that are a efficient catalyst for a biochemical reaction. It’s responsible to speed up reaction by having an alternative pathway of low active energy. In a simple cell it can be found 2000 different enzymes, each one of these have the ability on catalyzing a different reaction. Each of the enzyme found are responsible on having a reaction occurring in the cell. In this certain reaction in the cell, the cell will tend to make poisonous chemical that could be harmful to the cell itself, the enzyme job here is to be used to breakdown these poisonous chemical to a harmless substance. Catalese enzyme is a common enzyme that is found almost in all living organism including liver. This enzyme present in a liver exposed to oxygen
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A catalese enzyme are exist in a certain living organism which in this experiment will be using chicken liver to help study the catalese that contain in the liver cell easily. The catalese again, will breakdown harmful hydrogen peroxide into a oxygen and water (2H2O2 ----> 2H2O + O2). When this reaction occurs in the experiment, then it will be seen that oxygen gas bubbles escape and create foam like. From here, we would like to see how temperatures that occur in the environment affect a reaction of an enzyme towards a substrate.
Research question
How does different temperature (20°C, 30°C,40°C,50°C,60°C) affect the catalese enzyme reaction rate present in a liver, having a substrate as hydrogen peroxide, in terms of heights of bubbles in cm in 20 second?
Hypothesis:
Temperature analyzes a measurement of degrees of coldness and hotness of the environment. In this experiment, if the temperature of the liver increases then the amount of the bubble that will produce will decreases, because from research when a protein within a food such as liver exposed to heat, it will cause the atoms that exist in it to work viciously and cause to break bonds with the protein, and there will be no longer reaction of a liver towards the hydrogen peroxide substrate. Hence, when the temperature increases, the height of the bubble will be
A catalese enzyme are exist in a certain living organism which in this experiment will be using chicken liver to help study the catalese that contain in the liver cell easily. The catalese again, will breakdown harmful hydrogen peroxide into a oxygen and water (2H2O2 ----> 2H2O + O2). When this reaction occurs in the experiment, then it will be seen that oxygen gas bubbles escape and create foam like. From here, we would like to see how temperatures that occur in the environment affect a reaction of an enzyme towards a substrate.
Research question
How does different temperature (20°C, 30°C,40°C,50°C,60°C) affect the catalese enzyme reaction rate present in a liver, having a substrate as hydrogen peroxide, in terms of heights of bubbles in cm in 20 second?
Hypothesis:
Temperature analyzes a measurement of degrees of coldness and hotness of the environment. In this experiment, if the temperature of the liver increases then the amount of the bubble that will produce will decreases, because from research when a protein within a food such as liver exposed to heat, it will cause the atoms that exist in it to work viciously and cause to break bonds with the protein, and there will be no longer reaction of a liver towards the hydrogen peroxide substrate. Hence, when the temperature increases, the height of the bubble will be