The crude extract containing a catalase, catalyse a reaction where the substance is simultaneously oxidized and reduced, giving two different products of H2O to O2 and H2O. This reaction was measured by UV-Vis spectroscopy which results a decrease in absorbance at 240nm. The decrease in absorbance was caused by the loss of H202 in a product. The catalase enzyme was purified by the 5 – step procedure using a sodium phosphate buffer and a DEAE- Sepharose column which is mainly used for protein purification. The results of catalase purification is shown in Table 4
25 mM of Sodium phosphate was added to the column to elute any an unbound protein it was collected to the Fraction B. To elute bound protein 65mM of phosphate buffer was added to the
25 mM of Sodium phosphate was added to the column to elute any an unbound protein it was collected to the Fraction B. To elute bound protein 65mM of phosphate buffer was added to the