Is it worth it to use Carnoy’s solution as a complementary therapy of benign odontogenic tumors treatment? An in vivo study in Wistar rats
Shot title: Carnoy’s solution effects in mandible of rats Summary
In order to quantify osteonecrosis, blood clot and bone neoformation after Carnoy’s solution (CS) and saline application in mandibles of Wistar rats, a monocortical defect was created bilaterally on right/left buccal cortical of Wistar rat’s mandibles and received one application of 5 minutes of CS and one application of 5 minutes of saline, respectively. This procedure followed the standard recommendations of CS use as a complementary therapy of benign odontogenic tumor treatment. Fifteen animals were equally divide in three groups for …show more content…
Immediately and after three days of its application, CS did not show to cause more osteonecrosis on osseous bed of monocortical defects than saline solution. However, after three days, amount of blood clot was significant more for control specimens (p = 0,019).Ten days after applications, amount of bone neoformation was not different between CS and saline (Table 1). Intergroup comparisons for necrosis, blood clot and inflammation demonstrated that three days after applications, CS and saline caused more necrosis, blood clot and inflammation than samples collected immediately after saline application (necrosis, p = 0,045 and 0,018; blood clot, p = 0,019 and 0,005; inflammation, p = 0,004 and 0,005 for CS and saline, respectively - Tables 2 and 3). …show more content…
Applying CS on osseous bed after lesion removal would fixate depth localized tumor cells by causing an a bone penetration, commonly known as osteonecrosis, of 1,54 mm depth after five minutes exposure. We did not measure how profound was osteonecrosis but our findings suggest that CS do not cause this phenomenon sufficiently to promote a satisfactory safety margin to surgery when compared to saline, an inert substance. Maybe a cadaveric bone8 responds different to solution than a viable bone. Even with CS causing more necrosis on osseous bed three days after its application than after immediate saline application, three days after its application, saline also did so. These findings suggest that necrotic bone fragments encountered on bone surface three days after CS and saline applications may be attributed to a physiologic process of wound
Shot title: Carnoy’s solution effects in mandible of rats Summary
In order to quantify osteonecrosis, blood clot and bone neoformation after Carnoy’s solution (CS) and saline application in mandibles of Wistar rats, a monocortical defect was created bilaterally on right/left buccal cortical of Wistar rat’s mandibles and received one application of 5 minutes of CS and one application of 5 minutes of saline, respectively. This procedure followed the standard recommendations of CS use as a complementary therapy of benign odontogenic tumor treatment. Fifteen animals were equally divide in three groups for …show more content…
Immediately and after three days of its application, CS did not show to cause more osteonecrosis on osseous bed of monocortical defects than saline solution. However, after three days, amount of blood clot was significant more for control specimens (p = 0,019).Ten days after applications, amount of bone neoformation was not different between CS and saline (Table 1). Intergroup comparisons for necrosis, blood clot and inflammation demonstrated that three days after applications, CS and saline caused more necrosis, blood clot and inflammation than samples collected immediately after saline application (necrosis, p = 0,045 and 0,018; blood clot, p = 0,019 and 0,005; inflammation, p = 0,004 and 0,005 for CS and saline, respectively - Tables 2 and 3). …show more content…
Applying CS on osseous bed after lesion removal would fixate depth localized tumor cells by causing an a bone penetration, commonly known as osteonecrosis, of 1,54 mm depth after five minutes exposure. We did not measure how profound was osteonecrosis but our findings suggest that CS do not cause this phenomenon sufficiently to promote a satisfactory safety margin to surgery when compared to saline, an inert substance. Maybe a cadaveric bone8 responds different to solution than a viable bone. Even with CS causing more necrosis on osseous bed three days after its application than after immediate saline application, three days after its application, saline also did so. These findings suggest that necrotic bone fragments encountered on bone surface three days after CS and saline applications may be attributed to a physiologic process of wound