Effects of Different Concentrations of Caffeine on Cellular Respiration in a Saccharomyces Cerevisiae Cell
Valentine Bedford
Red Deer College- Red Deer
Results
The negative control showed no release of CO2 gas. The positive controls (0.1 and 0.5) showed positive results that the caffeine did not inhibit, but increase the CO2 amount measured in the Durham tubes. The CO2 gas released peaked at the 1.5 mL concentration of caffeine, but dropped when the caffeine concentration increased to 2.0 mL. The greatest amount of caffeine concentration inhibited the cellular respiration in a Saccharomyces Cerevisiae cell.
Table I. Concentrations of Caffeine Solutions
Concentration (mL) 0 0.05 0.1 0.5 1.0 1.5 2.0
Average CO2 Released (mL) 0 3.89 3.92 3.52 4.70 4.88 4.46
Standard Deviation 0 0.91 0.61 0.45 0.30 0.10 0.25
Figure 1.
Discussion
The aim of this study was to see if caffeine affected cellular …show more content…
A determinate error that would make our results shift higher would be not subtracting the air already in the Durham tubes before they were placed in the water bath when measuring the CO2 gas released, as well as not being fast enough when transferring the yeast-caffeine solutions into the Durham tubes. More variance in the concentrations of caffeine solutions would help pin point exactly were the drop in cellular respiration happened to examine exactly how much caffeine could be taken while still being beneficial as a higher concentration would lead to the caffeine to no longer be beneficial or be in excess.
Literary Citations
1) Shearer, J. Graham, T E. (2014) Performance effects and metabolic consequences of caffeine and caffeinated energy drink consumption on glucose disposal. Nutrition Reviews 72 121–136
2) Nehlig, A. Boyet, S. (2000) Dose–response study of caffeine effects on cerebral functional activity with a specific focus on dependence. Brain Research 858
Valentine Bedford
Red Deer College- Red Deer
Results
The negative control showed no release of CO2 gas. The positive controls (0.1 and 0.5) showed positive results that the caffeine did not inhibit, but increase the CO2 amount measured in the Durham tubes. The CO2 gas released peaked at the 1.5 mL concentration of caffeine, but dropped when the caffeine concentration increased to 2.0 mL. The greatest amount of caffeine concentration inhibited the cellular respiration in a Saccharomyces Cerevisiae cell.
Table I. Concentrations of Caffeine Solutions
Concentration (mL) 0 0.05 0.1 0.5 1.0 1.5 2.0
Average CO2 Released (mL) 0 3.89 3.92 3.52 4.70 4.88 4.46
Standard Deviation 0 0.91 0.61 0.45 0.30 0.10 0.25
Figure 1.
Discussion
The aim of this study was to see if caffeine affected cellular …show more content…
A determinate error that would make our results shift higher would be not subtracting the air already in the Durham tubes before they were placed in the water bath when measuring the CO2 gas released, as well as not being fast enough when transferring the yeast-caffeine solutions into the Durham tubes. More variance in the concentrations of caffeine solutions would help pin point exactly were the drop in cellular respiration happened to examine exactly how much caffeine could be taken while still being beneficial as a higher concentration would lead to the caffeine to no longer be beneficial or be in excess.
Literary Citations
1) Shearer, J. Graham, T E. (2014) Performance effects and metabolic consequences of caffeine and caffeinated energy drink consumption on glucose disposal. Nutrition Reviews 72 121–136
2) Nehlig, A. Boyet, S. (2000) Dose–response study of caffeine effects on cerebral functional activity with a specific focus on dependence. Brain Research 858