The preparation for this lab should be done a week prior to examining each layer. The preparation consists of compiling the pond. To do so, obtain a small, disposable bowl with a cover. Using a thumbtack, poke holes into the lid of the container. Then, using black paper, wrap it 2/3 of the way around the bowl and proceed to the securing the black paper with aluminum foil.…
The growth of the buds was recorded and put in graphs. Each group obtained a pot from the instructor. After obtaining a pot, labels were applied and written with a ball point pen. Each label had the time and written on it. A small section of folded paper towels was inserted over the holes in the bottom of the pot.…
The procedure for Lab 5 contained four parts. For Part A, a clean, dry 100 mL beaker was obtained and filled with 60 mL of distilled water. Next, the beaker was placed on a ring stand with a thermometer attached to the ring stand to record the temperature of the water. After the thermometer had been in the water for two minutes, the temperature was recorded. Following the recording of the temperature, a 10 mL pipet and a bulb was used to pipet 10.00 mL of distilled water into a clean, dry, pre-weighted 50 mL beaker.…
The First Source reviewed was an article from the website Education.com called Leaf Chromatography by Beth Touchette. In the Article Leaf Chromatography Beth Touchette discusses the “how to’s” on the project itself and important facts on Leaf Chromatography. First, this article explores photosynthesis and the pigments such as chlorophyll, carotenoid, and anthocyanin and explains their colors and properties. The reading also includes such advice on what to do during project like always check the filter paper every minute or so or instead use coffee filter paper for the project.…
Purpose: To observe different species of living protists in pond water under the microscope. Taking note of their size, movements, and characteristics to classify the different types of protists from applying previous knowledge of protists. Materials: The materials in this lab that will be needed are a microscope, slide, coverslips, a pipette, and a dish of pond water. Procedure: Prepare a slide by using the pipette to apply two drops of pond water on a slide and properly place a coverslip.…
The carrot you only need a small piece of while the grapes you will need two to three and a handful of spinach leaves. Next you will need a triple beam balance to measure the mass of the items, a knife to cut the items and a large paper towel. Once you have all the materials you can begin the experiment. First make a table five by four table to record your data. Have four columns for the mass on the first day, mass on the third day, the difference between the two and the percentage of water found.…
It is important to note that the goal of this experiment was to find the pKa value of bromothymol blue, a pH indicator commonly used in titrations. [UNC, 2017] The pKa is where the number of moles of acid to conjugated base are equivalent. To find this, both Beer’s Law and spectroscopy were utilized in an attempt to make graphs to show just where this value occurred. According to Sigma-Aldrich, the pKa of bromothymol blue is approximately 7.10.…
The experiment came out successful based on the results. We did encounter one problem with one of the solutions. The colorimeter results for Biscayne Bay Pond Water didn’t come out right; so we had to dilute the solution to get the result. Analysis: First turn on the colorimeter and select “ALL TESTS” from the testing menu. Press “AMMONIA-N H” from the menu.…
A colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich Chemical Co.) assay was performed to measure the cell viability. Briefly, RAW 264.7 cells were treated with different concentrations of spermidine for 24 h or pretreated with spermidine for 1 h before stimulation with LPS for 24 h. After incubation, the medium was discarded, and MTT solution (5 mg/mL in phosphate-buffered saline, PBS) was added to each well and incubated for another 3 h at 37 °C. The medium was removed, and DMSO was added to dissolve the formazan dye. The optical density was then read at 560 nm using a microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA) to determine the cell viability. Measurement of NO production…
Confirmation of Mendel’s Law of Segregation I. In Biology class the past few weeks we have been learning about Gregor Mendel. Gregor Mendel is often called the father of modern genetics. Mendel developed the theory of inheritance several decades before the observance of chromosomes via microscope. In Biology lab the past two weeks I completed a series of tests using fast plants, Brassica rapa.…
its color led to the formulation of this experiment. The experiment utilizes chloroplasts of spinach leaves to test the different color lights’ effects on photosynthesis. Green light is said to be the least effective at driving photosynthesis on the electromagnetic spectrum due to being mostly reflected and transmitted instead of being absorbed. Conversely, red light and white light is more effective at driving photosynthesis (Reece, 2011). Thus, a hypothesis was created stating that the rate of photosynthesis will be greater with a red light bulb compared to a green or white fluorescent light bulb.…
The purpose of the Light, Color, and Solutions experiment was to determine how to use a spectrophotometer, understand the relationship between a solution’s visible color and its color absorption, and to derive, understand, and use Beer’s Law. Some background information that would be helpful included that the color of a solution was determined both by the colors it absorbed as well as the colors it transmitted. For example, if a solution was viewed as the color red, then red light was transmitted by the solution while the complementary color, green, was absorbed. Beer’s Law stated that the amount of light that passed through a sample was limited by the light’s wavelength,…
Control Variables: Amount of dirt, time in light, type of bean, type of soil, source of the light, amount of liquid, room temperature, and time watered. Dependent Variables: Height of plant, # of leaves on the plant, posture of the stem. Independent Variable: Creek water and miracle grow + water Procedure: Experimental Group: Plants watered with creek water and miracle grow + water Control Group: Plant watered with tap water Grab three cups and three lima bean seeds Label one cup tap water, one creek water, and one miracle grow + water Fill the cups up with the same potting soil, half full Bury the seeds under the dirt, about an inch down Water the seeds with 25 milliliters of the designated liquid in a graduated cylinder For the miracle grow, fill the graduated cylinder with 25 milliliters of water, then put in a pinch of fertilizer, and mix Measure all the plants height (with a ruler), check the posture of the stem, and number of leaves Record your data into a data table Put your plants back under the…
Purpose The purpose of this experiment is to find the pKIn blue using spectrophotometry. Bromophenol blue dissociate in water by the following reaction: i) HIn + H2O ⇋ H3O+ + In- The KIn value can be calculated from the reaction: ii) K_In=[H3O+]…
Start of the process of setting up the experiment, by adding 400 millilitres of water at a temperature of about 32°C into the beaker. Have a timer…