Bromophenol Blue Lab Report

Decent Essays
Bromophenol Blue is a common used pH indicator dye and is with low pKa (Andrew 1995). As an acid-base indicator, bromophenol blue will lose a proton when pH of the environment around this indicator is higher than the pKa of the dye. This displacement changes the electronic distribution within the molecule and the indicator changes its color from yellow to blue (Liam, 2003). Filter paper is used to absorb bromophenol blue solution to make BPB indicator strips. After strips are exposed to the headspace of samples, bromophenol blue will react with volatile biogenic amines and change color from yellow to blue color. Colorimeters is a color measurement instruments and is used to measure the indicator strips. L*a*b* system is a cylindrical coordinate

Related Documents

  • Superior Essays

    Purpose and Background Cells divide in two ways, mitosis and meiosis. Mitosis is used to produce cells that are genetically identical to the parent cell for growth, asexual reproduction, or repair after injury. Cells that are produced by mitosis are diploid, meaning that they have two complete sets of chromosomes, one from each parent. Meiosis is used to produce haploid cells that have only one set of chromosomes, a mix of chromosomes from both parents. Meiosis produces cells that are genetically unique from their parent cells.…

    • 1551 Words
    • 7 Pages
    Superior Essays
  • Improved Essays

    Interactive Question 7.2 Cite some experimental evidence that indicates that membrane proteins drift. A good form of experimental evidence is Fluorescence Recovery After Photobleaching, aka FRAP. In the FRAP process, membrane proteins are labelled with a green fluorescent protein, and part of the plasma membrane is “bleached” with a laser, causing them to lose their fluorescence. The part that was bleached will eventually become fluorescent again, as molecules drift in by diffusion.…

    • 853 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Goals The goal of the lab was to investigate the properties and structure of an unidentified compound that was discovered in a local landfill. The group was called in to help determine the chemical and physical properties of the compound so we can let the people of the town know so it can be taken care of properly with or without further precautions to be taken. Another goal of this project was to devise the synthesis of the unknown compound. Experimental Design In order to determine the correct identity of our unknown compound, we first started with a couple preliminary tests, which include physical state and smell.…

    • 757 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    The colours displayed on the strips were held against the colours on the box and the pH was recorded. With the…

    • 205 Words
    • 1 Pages
    Improved Essays
  • Improved Essays

    Red Dye 2 Lab Report

    • 841 Words
    • 4 Pages

    Red Dye 1 Red dye 1 is most likely a polar covalent molecule since we have found that it dissolves into water but does not break apart into ions. Just because it dissolves in water does not make it solely polar covalent, but since it has a relatively low conductivity, 534 mS/cm in the 0-20000 range, then it most likely is polar covalent. This is further confirmed with the fact that it does not react with NaOH, NaCl, or AgNO3, all ionic substances. The red dye does not react with NaOH since there was no color change when added, and the conductivity simple rose as the NaOH was added, due to the fact the excess ions were being added to the solution and not reaction at all.…

    • 841 Words
    • 4 Pages
    Improved Essays
  • Decent Essays

    Within methods of chemistry, the most common and obvious way to find the PH of a substance is to use an acid-base indicator in order to determine the pH level by comparing the indicators color to what pH it represents. An example of this is universal indicator (a mixture of a variety of other indicators) that changes a wide variety of colors depending on the pH it is subject to. This colorizing effect is somewhat like a color dye for a molecule, that changes color when it detects a specific threshold is crossed in terms of the pH of the substance, thus allowing an approximate pH to be determined. This color change is due to the molecules present in acid-base indicators responding to the changes in the overall concentration of the hydrogen…

    • 140 Words
    • 1 Pages
    Decent Essays
  • Improved Essays

    Module 1 Lab Report

    • 386 Words
    • 2 Pages

    Nettie Stauts January 26, 2018 CDT-2413 Module 1 Lab Parents Perspective: The parents were apprehensive, but eager, entering their son Luke in a child care program. They were getting early intervention home-centered services in which Luke had progressed with assistance from his therapist and babysitter. He lacked social developmental skills and they heard about inclusion although unfamiliar with the concept. They were adapted interacting with Luke through gestures and augmentative communication devices yet anxious about his communication in the classroom.…

    • 386 Words
    • 2 Pages
    Improved Essays
  • Improved Essays

    Identifying Unknown Substance. Examining the physical characteristics of the available compounds in the lab was how possible compounds were eliminated from any or further testing. Based on how the compounds physically looked, it was almost for certain that calcium chloride was the unknown compound. However, another compound was used to test as well (ammonium chloride) since relying only on physical characteristics alone would not allow for proper experimentation. The unknown ended up being calcium chloride, however, until the pH test, ammonium chloride was still a possibility because the ammonium chloride could have been ground up.…

    • 511 Words
    • 3 Pages
    Improved Essays
  • Improved Essays

    Phenol is a poisonous crystalline acidic that presents itself as tars of coal and in wood. In dilute solution it is use as an disinfectant. When the peroxy acid ester and phenol mix it releases energy that excites atoms in the fluorescent dye and causing photons which are released particles of light. Glow sticks has two counters.…

    • 662 Words
    • 3 Pages
    Improved Essays
  • Decent Essays

    Cyclohexanone Lab Report

    • 173 Words
    • 1 Pages

    This week’s lab focused on a green chemistry reaction of cyclohexanol and acetic acid, resulting in the formation of cyclohexanone. The identity of the product was confirmed using an IR spectrum to determine the major functional groups of the compound. The two main functional groups that appear in the spectrum are the C-H stretch around 3000 cm-1 and the C = O stretch around the 1600-1700 cm-1.…

    • 173 Words
    • 1 Pages
    Decent Essays
  • Improved Essays

    Buddy Lab Report

    • 795 Words
    • 4 Pages

    Instructions: Buddy Lab - Physical and Chemical Change You’ve learned a lot about matter lately and now is your chance to teach someone younger about what you know. Your job is to design a lab that will help a primary student experiment and understand what physical and chemical changes are. Follow the guiding questions below to help you create your lab. Question:…

    • 795 Words
    • 4 Pages
    Improved Essays
  • Decent Essays

    It is important to note that the goal of this experiment was to find the pKa value of bromothymol blue, a pH indicator commonly used in titrations. [UNC, 2017] The pKa is where the number of moles of acid to conjugated base are equivalent. To find this, both Beer’s Law and spectroscopy were utilized in an attempt to make graphs to show just where this value occurred. According to Sigma-Aldrich, the pKa of bromothymol blue is approximately 7.10.…

    • 632 Words
    • 3 Pages
    Decent Essays
  • Improved Essays

    Bouncy Test Lab Report

    • 657 Words
    • 3 Pages

    Bouncy balls bounce because the material of the ball is extremely elastic and can convert kinetic energy from falling into potential energy and back into kinetic energy again with little loss of momentum. They are used in many classrooms because they can easily demonstrate conservation of momentum through a highly elastic collision. Like many things made out of rubber, bouncy balls have very high elasticity, meaning they rapidly return to their resting state after being stretched or squashed.…

    • 657 Words
    • 3 Pages
    Improved Essays
  • Decent Essays

    Questions 1. Why are three replicate titrations done for each sample? Three titrations are performed because an error may occur in one of the titrations and getting two extra titrations can help eliminate the wrong answer. It is also a great way to practice accuracy and precision.…

    • 256 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    Does a Measurement Result in a True Value? Limitations of Measurements Elizabeth Lechtholz-Zey & Marisa Loredo 10/5/15 CHEM 101-08 Purpose To determine the differences in precision and accuracy in weighing 10 mL of water using a 50 mL beaker, a 10 mL graduated cylinder, and a 50 mL buret. Data Temperature of water: 23.0ºC 100 mL beaker weight: 50.557 g # of additions of water to the 100 mL beaker * 50 mL beaker (±5 mL) * 10 mL graduated cylinder (±0.05 mL) * 50 mL buret (±0.05 mL) 0 0.00 g 0.00 g 0.00 g 1 7.48 g 9.91 g 9.95 g 2 14.23 g 19.70 g 19.83 g 3 21.38 g 29.56 g 29.85 g 4 29.20 g 39.44 g 39.77 g 5 35.91 g 49.30 g 49.72 g * 10 mL graduated cylinder (±0.05 mL) 0 50.557 g 1 60.340 g 2 70.010…

    • 1103 Words
    • 5 Pages
    Improved Essays