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30 µl measurements of 0.4, 0.8, 1.2, 1.6 and 20 mg/ml were added into their own sample of the 3 ml Bradford Assay, 30 µl of water was added to a separate sample of Bradford Assay and 30 µl of the dilute liver homogenate was added to its own sample of Bradford Assay and left for 3 minutes. The absorbance of the standards and the diluted homogenate samples were recorded to create a standard curve. From this equation, the protein concentration of my sample was determined. Class averages were also obtained (Clendening).
Y= 0.52x + 0.186 , R2= 0.9572
Protein to DNA ratio was calculated by dividing the determined average protein concentration of the homogenate by the given DNA concentration for all three Bos taurus homogenates (Clendening).Original homogenates were used to digest Glycogen. First, 50 µl of 8 mg/ml amyloglucosidase was added to 50 µl of homogenate to create a …show more content…
Here we see that the kidney homogenate had the highest concentration of triglycerides followed closely by the liver homogenate.
In order to obtain values for glycogen, free glucose and total glucose concentrations had to be calculated using the glucose standard curve. Once these numbers were known, subtracting free glucose from total glucose came out to the amount of glycogen. Figure 4 shows free glucose levels, where the liver had the highest amount and the kidney the lowest. Figure 3 provides glycogen concentration, where again the liver has the highest levels, but the heart homogenate has the lowest.
To conclude, each cell type of the Bos taurus had varying levels of macromolecules according to the experiments performed in these
Y= 0.52x + 0.186 , R2= 0.9572
Protein to DNA ratio was calculated by dividing the determined average protein concentration of the homogenate by the given DNA concentration for all three Bos taurus homogenates (Clendening).Original homogenates were used to digest Glycogen. First, 50 µl of 8 mg/ml amyloglucosidase was added to 50 µl of homogenate to create a …show more content…
Here we see that the kidney homogenate had the highest concentration of triglycerides followed closely by the liver homogenate.
In order to obtain values for glycogen, free glucose and total glucose concentrations had to be calculated using the glucose standard curve. Once these numbers were known, subtracting free glucose from total glucose came out to the amount of glycogen. Figure 4 shows free glucose levels, where the liver had the highest amount and the kidney the lowest. Figure 3 provides glycogen concentration, where again the liver has the highest levels, but the heart homogenate has the lowest.
To conclude, each cell type of the Bos taurus had varying levels of macromolecules according to the experiments performed in these