Ir Spectrophotometry Lab Report

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Introduction
There are many bioactive compounds that can be found around us. Bioactive compounds mostly can be found in both plants and animals. It is a compound that has an effect on a living organism, tissue or cell. It also can have an influence in our health. A few examples of bioactive compounds included flavonoids, caffeine, carotenoids, carnitine, choline, coenzyme Q, creatine, dithiolthiones, phytosterols, phytoestrogenss, glucosinolates, polyphenols, prebiotics, and taurine. Carotenoids, polyphenols and phytosterols are examples of plant bioactive compounds. Carotenoids and polyphenols are originated from vegetables and fruits while phytosterols is from oils. Fatty acid is the example of animal bioactive compounds which are found in
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In this review, only the spectrophotometric method is discussed. The spectrophotometry is a method that involve in infrared spectrum and a quantitative measurement of the reflection of a material and it represent as a function of wavelength. Thus the infrared spectroscopy (IR spectroscopy) is the spectroscopy that deals with the infrared region of the electromagnetic spectrum that is, light with a longer wavelength and lower frequency than visible light. Infra-red spectrum is usually divided into 3 spectral regions which are near infra-red, middle infra-red and far infra-red regions. Carotenoids absorb light in the 400-500 nm region of the visible spectrum. This physical property imparts the characteristic yellow-red colour of the …show more content…
( Source: Oliver & Palou, 2000).
Carotenoids have a lot of benefits in human health. Its biological functions have attributed to these compounds. Carotenoids plays an important nutritional role as the precursor of vitamin A as shown by beta-carotene. These principal precursor of vitamin A help in preventing blindness and eye problems, cell differentiation, synthesis of glycoprotein, mucus secretions from epithelial tissues, overall growth, development of bones as well as in reproduction. The benefits of carotenoids can be summarized into pro-vitamin A, immune enhancement, prevention of mascular degeneration, inhibition of cancer, prevention of cardiovascular disease and deceased risk of cataract formation (Cuttriss et al.,2011). Although the other type of carotenoids which are mostly occur in green leaves lacking in pro-vitamin A, they are still important to human health as they may have relation to cancer prevention.
2. Fourier transforms infrared spectroscopy

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